Replication Requirements
DNA replicates in this manner, that allows for 50% more to be produced after the first round.
Semi-conservative(ly)
What molecule is needed to start the whole shebang?
Initiator Protein
what structure protects genetic material by containing it?
Telomere
Which protein 'untangles' the DNA after helicase elongates it?
gyrase
What breaks the hydrogen bond between base pairs during replication?
Helicase
Replication requires these 3 fundamental components to occur.
A template
Raw Materials
Enzymes to carry out reactions
What does the initator protein give insturctions to do?
To start synthesizing from this start point
Mismatched base pairs, deformities in secondary structure
Which protein synthesizes the majority of DNA?
DNA Polymerase III
This refers to the direction that DNA is synthesized
5'-3'
This refers to the direction that DNA is read
3'-5'
What stabilizes the strands of DNA after they've been separated?
single-strand binding proteins
True or false. Termination can occur in multiple ways.
True
Which proteins proofread the DNA?
DNA polymerase I & III
What are the four phases of replication?
elongation, unwinding, termination, & initiation
The lagging strand is inhibited by which fundamental component of replication not being met
No template to read (runs out)
Which need more energy to break bond between?
A-T or C-G?
C-G
What is the end replication problem unique to? Who/What does it affect?
Eukaryotes
What is the order for these 3 proteins to occur?
DNA polymerase I, helicase, Ligase
helicase, DNA polymerase I, ligase
What makes origins of replication good places to start synthesis?
Rich in A-T base pairs
Name each kind of replication between Eukaryotes and prokaryotes.
Euk-Linear replication
Pro-Theta/rolling circle
The replication bubble expands in which direction?
Bi-directionally
What is the end replication problem?
Primers at ends of chromosomes cannot be replaced and so chromosome shortening occurs.
This molecule makes RNA primers necessary for DNA polymerase to bind to starting points
primase
Who discovered Ozaki fragments?
A dude with the last name, Ozaki.