Lab Techniques
The movement of molecules in a gel under influence of an electric charge.
What is electrophoresis?
Pipettes that dispense a specific amount of liquid through a plastic tip.
What is a micropipette?
The bacteria being transformed in this lab.
Plasmids consisting of DNA from multiple different sources.
What is recombinant DNA?
The monomer that makes up a protein.
What is an amino acid?
Practices under sterile conditions that prevent contamination with microorganisms.
What is aseptic technique?
Protective clothing or equipment that is worn to prevent infection due to microorganisms.
What is PPE (personal protective equipment)?
The process of a cell taking up DNA from its surrounding environment.
What is bacterial transformation?
pGFP contains resistance to this antibiotic.
What is ampicillin?
What is GFP (green fluorescent protein)?
The process of allowing microbial organisms to reproduce in a medium under controlled conditions.
What is cell culturing?
A rapidly rotating machine that separates different substances of different densities.
What is a centrifuge?
What is bacterial conjugation?
A specialized type of plasmid containing an origin of replication, cloning cites, promoters, and selectable markers.
What is a vector?
The protein that provides resistance to ampicillin.
What is beta-lactamase?
The separation of a mixture by passing it through a solution that its components pass through at different rates.
What is chromatography?
A device used to thoroughly mix solutions rapidly.
What is a vortex mixer?
A buffer solution that breaks open cell walls.
What is lysis buffer?
What is the T7 promoter?
The type of electrophoresis used to analyze proteins.
What is PAGE (polyacrylamide gel electrophoresis)?
Quickly moving cells between low and high temperatures to alter the permeability of the cell walls.
What is heat shock?
The tube in which the chromatography is performed in.
What is a column?
Cells that have the ability to take up extracurricular DNA to alter their genotype.
What are competent cells?
The inducer that inactivates lac repressors, allowing for T7 polymerase to be expressed.
What is IPTG?
A detergent used to disrupt interactions between amino acids.
What is SDS (sodium dodecyl sulfate)?