Blast From the Past
Glioma is presented in this nervous system
What is Central Nervous System
This blotting is used for protein analysis
What is Western Blot?
This treatment uses radiation to increase amounts of CD133+
What is Ionizing Radiation (IR)?
This animal was used for this experiment
DAILY DOUBLE
What are Mice?
Glioma kills this certain amount of people per year
What is 16,000?
Fluorescent in situ Hybridization
When FISH was used CD133+ had this characteristic
What are Cancer Stem Cells?
This characteristic was given due to CD133+ cells quickly repairing damaged DNA
What is Radio Resistance?
This WHO grading has low-level mitotic activity
What is Grade II?
A subset of glioma cells that have CD133
DAILY DOUBLE
What are Neurospheres?
These two proteins used with CD133+ lead to an increased amount of CD133+ (Western Blot)
What is Chk1 and Chk2
These genetic markers [name at least two :)]
What is CD133, Sox2, Musashi, and Nestin?
Stem cells in Glioma originate from these two regions in an adult brain
What is Subventricular Zone and Subgranular Zone
DNA comes out of the cell during Comet Assay due to
What is Lysing?
The decreased activation of this protein lets CD133+ have low rates of apoptosis
What is Caspase-3?
This pathological marker has weirdly shaped nuclei (psst its from lecture slide)
What is Atypical Cellular Appearance?
These Three Central Nervous System tumors
DAILY DOUBLE
What is Astrocytoma, Oligodendroglioma, and Glioblastoma?
Colony Formation Assay measures
What is Cell's Survivability?
CSFE and CMTRX assisted with Annexin V staining in determining this amount of fold
What is four-fold?
This term is used to describe the capability of forming tumors
What is Tumorigenic?