DNA
The current in a gel electrophoresis runs in this direction
What is positive to negative
The buffer we use for DNA gel electrophoresis
What is TAE
The first thing you do when you need to work with a new chemical
What is, read the SDS
500mL of 1X TAE from 50X TAE
What is 10mL of 50X TAE and 490mL of DI water
What is the Chickadee
This is used for determining unknown lengths of DNA fragments in a gel by comparison and distance traveled
What is the DNA Ladder
The enzyme used to replicate DNA in PCR
What is Taq polymerase
The most common exposure to ergonomic stress injuries
What is a micropipette
500mL of 1X FastBlast from 500X Fastblast
What is 1mL of FastBlast with 499mL of DI water
The two sports that were invented in Springfield and Holyoke.
What is Volleyball and Basketball
Why would we use a 3% gel over a 1% gel?
What is, when the fragments are smaller
A bacteriophage commonly used as template DNA
What is Lambda DNA
This needs to be completed prior to initiating a laboratory procedure
What is the protocol
The quantity of water to put in tubes to create a 1mL, 4-step 1:10 serial dilution using yeast.
What is 900uL
The second largest city in New England by population
What is Worcester, MA
This was the first restriction enzyme isolated from e. coli
EcoRI
An apparatus which allows to raid heating or cooling of samples at specific time frames for a specific quantity of cycles
What is a thermocycler
The most effective control strategy for minimizing risk in a laboratory
What is Elimination
The quantity of water to put in tubes to make a 1mL, 4-step, 1:2 serial dilution
What is 500uL
Fig Newtons where named after this MA city where they were in invented
What is Newton
This molecule searches for a specific palindromic sequence in a DNA and then cuts at a specific spot to create smaller fragments of DNA
What is a Restriction Enzyme
A plate or chip coated with cDNA which allows for rapid testing for many genetic indicators.
What is a microarray
The correct order of safety controls from most effective to least effective.
What is, Elimination, Substitution, Engineering Controls, Administrative Controls, PPE
Making a 50mL, 0.2M NaOH solution from 1.0M solution.
What is 10mL of 1.0M NaOH and 40mL of DI water
Banned to include in clam chowder
What is tomatoes