GRIFOLS ERYTRA EFLEXIS TRAINING

Reagents and Samples.

100

What temperature should your Gel Cards Be Stored?

Room Temperature. 

200

How many wells are in a standard Grifols DG Gel Card?

8 Wells! DG GEL 8. It's in the name, amazing marketing Right?!

300

Match the Reagent or Solution to its description/function.

Grifols Wash Solution A 

Grifols Wash Solution B 

Grifols Diluent 

Red Blood Cell Reagents 

A. Used to prepare human red blood cell suspensions.

B. Detergent-based cleaning solution for the analyzer fluidic system.

C. Used to detect human blood group antibodies.

D. Saline-based cleaning solution for the analyzer fluidic system.

Grifols Wash Solution A - D. Saline Based Solution

Grifols Wash Solution B - B. Detergent Based Cleaning Solution

Grifols Diluent - A. Used to Prepare Human RBC Suspensions

Red Blood Cell Reagents - C. Used to Detect Human Blood Group Antibodies

400

What is the correct dilution ratio for your Grifols Wash Solution w/ DI Water? 

1:16 

Dilute one bottle (125 mL) of concentrated Grifols Wash Solution with 1,875 mL de-ionized (DI) water to produce 2 L diluted wash solutions (1:16 dilution) before use with the analyzer. Each container can hold up to 6 L of diluted wash solution. 

Did you Know? The analyzer can hold up to 4 wash solution containers when it is connected directly to a laboratory drain for solution waste disposal.

500

Prior to use, Red blood cell reagents must be ____ and then uncapped immediately prior to use.

Prior to use, Red blood cell reagents must be GENTLY SUSPENDED/INVERTED and then uncapped immediately prior to use.

600

A minimum sample volume of ____ is required for the analyzer to detect blood inside the tube.

A minimum sample volume of 250 μL is required for the analyzer to detect blood inside the tube. For whole blood samples, the volume of plasma above the surface of the red cell layer must be sufficient to perform the desired number of plasma tests.

700

Determine if the sample is either acceptable  or unacceptable for use in the Eflexis Analyzer:

EDTA pink top tube.

Serum from a gel serum separator tube.

Whole blood sample tube before centrifugation.

Sodium Citrate tube without a barcode.

Serum only, from a red top tube.

Centrifuged whole blood sample collected in a red top tube.

EDTA Pink Top Tube (Acceptable)

Serum From a Gel Serum Separator Tube (

Whole Blood Sample Tube before Centrifugation (Acceptable)

Sodium Citrate Tube without Barcode (Acceptable IF you Manually Type and Scan the Sample)

Serum Only, From a Red Top Tube (Acceptable)

Centrifuged Whole Blood Sample Collected in a Red Top Tube (Unacceptable)

Start Me Up

100

Start up the analyzer in the following order:

First, switch on ____. 

Second, press ____. 

Start up the analyzer in the following order:

First, switch on the main power button on the back of the analyzer.

Second, press the start button on the analyzer. 

The Eflexis Analyzer is designed to remain powered on at all times, even when not in use. However, in some instances, the operator may need to turn the analyzer on and log in to the system.

-Make sure all analyzer doors and drawers are closed.

-Confirm that the main power switch located on the back of the analyzer is on.

200

Before Running your Analyzer, There are 5 containers that you need to check before loading samples. They are...

- Wash Solution A

- Wash Solution B

- Waste Container 1 

- Waste Container 2

- Solid Gel Card Waste Container 

300

True or False: You can remove liquid waste, solid waste, and wash solution containers when the lights are off.

False. Containers should only be removed when the LED lights are on.

The Blue Lights Need to Be on to Access A Specific Drawer. When lights are off, something is occurring in that reagent/waste container. 

400

Before Use of Your Analyzer, Where do you access on the software to check waste?

Before Use of Your Analyzer, Check these three places for Waste: 

In the CONTROLLER software, tap the STATUS tab, and then the CONTAINERS tab on the touchscreen display.

500

Part 1: How Do I Check the Gel Card Inventory on the Analyzer (250 points)? 

Part 2: How Many Gel Cards Can an Eflexis Hold in the Loading Station (125 points) and Part 2: Waste Container (125 points)?

Part 1 

In the CONTROLLER software, tap the STATUS tab, and then the CARDS tab on the touchscreen display Tap the Clipboard icon to view the type and number of gel cards currently on-board. Additional information may be obtained by tapping on the individual card racks.

Part 2 

200 Gel Cards 

Part 3 

200 Gel Cards

600

When you open and close the card station drawer, the card transport arm will automatically identify any new and previously loaded cards. The Card Transport Arm Performs the following tasks: (need to get three for points) . . .

• Detect the presence of DG Gel 8 cards

• Detect the number of cards

• Read the barcode of the 1st card

• Check expiration date and lot

• Update the information in the Status-Cards screen

700

Part 1: What are the two different sections blacked out (400 Points)?

Part 2: 

Describe the Locations in Section 1, 2, and 3 (100 Points Each)?

Part 1: 

Left Side: Non Agitated Reagents 

Right Side: Agitated Reagents 

Part 2: 

1. Locations for vials of reagents which require re-suspension.

2. Locations for vials of reagents which do not require re-suspension.

3. Location for a bottle of diluent or cleaner.

Standard Operation

100

Test profiles can be manually assigned using the ______ tab in the MANAGER software.

Once the Eflexis Analyzer detects the sample tubes, test profiles can be assigned manually using the WORKSHEET tab in the MANAGER software. 

- When Quality Control (QC) samples are loaded, they are detected by the analyzer and the required tests are automatically programmed.

-Any card or reagent onboard that does not have current QC associated with it will trigger a warning to the user.

200

When the analyzer is connected to an LIS, downloading test profiles can be done from the _______ tab.

When the analyzer is connected to a Laboratory Information System (LIS), downloading test profiles may be accomplished from the WORKSHEET tab. 

- If configured, Eflexis can automatically download test profiles from an LIS when samples are loaded.

To manually download a test profile from an LIS:

-In the MANAGER software, tap the Worksheet tab and then tap the Download from LIS icon on the right side of the screen.

300

Test results are categorized according to the following:

• Routine Results.

• Special Results.

• Results with Incidents.

• Results with Inconsistencies.

Give at least one definition of each and indicate the icon you'll see on the software.  

Routine Results are results that the Eflexis MANAGER software is able to interpret. Routine results do not require review before being validated. No Icon. 

Special Results occur When the MANAGER software is not able to provide an interpretation. Such as Special Microtube Results including Mixed Field, Doubtful Reaction Grade, and No Result Determined.

Results with Incidents are microtube incidents that occur during processing and affect the reaction grade. 

Results with Inconsistencies are discrepancies between two results for samples that have the same patient identification number or sample barcode.

400

Part 1 (True or False): Once the worksheet is populated with the desired profiles and test assignments downloaded from the LIS, the test process begins automatically (100 points). 

Part 2: (True or False) If Quality Control (QC) samples are on board and QC analysis is due, the Eflexis Analyzer will automatically begin processing the QC samples (100 points).

Part 3 (True or False): When it is time for the QC analysis, QC samples will be processed before routine blood samples (100 points).

Part 4 (True or False): QC analysis will be prioritized over STAT samples.

Part 1: True, The test process begins automatically once the worksheet is populated with the desired profiles and test assignments downloaded from the LIS. 

Part 2: True, The Analyzer will begin processing the QC samples automatically if the QC samples are on board and QC analysis is due (when Query by Sample is Activated).

Part 3: True, QC will be processed before routine samples. 

Part 4: False, STAT samples are always prioritized and will temporarily interrupt any QC analysis in progress.

500

100 Points for Each Color Match w/ the Sample/Profile Status Correctly Answered. 

600

Part 1: (True or False) Results with a White Eye icon have no incidents and can be validated without a detailed review (300 points).

Part 2: (True or False) Results with a Red Eye icon have incidents that require review, acceptance, and/or modification of the incident or special result for its validation (300 points).

Part 1: Results with a White Eye icon have no incidents and can be validated without a detailed review. (TRUE)

Part 2: Results with a Red Eye icon have incidents that require review, acceptance, and/or modification of the incident or special result for its validation. (TRUE)

700

Describe the information that is included for each report option:

-Results List Report 

-Reading Report

-Traceability Report

-Results List Report: 

Includes all of the results of the sample profiles selected, with the final interpretation of the results for each profile test.

-Reading Report: 

Includes all of the results of the sample profiles selected, with the final interpretation of the results for each profile test, along with the reaction grade associated with each microtube.

-Traceability Report: 

Includes all of the results of the sample profiles selected, along with the image of the processed card wells and the reaction grade associated with each microtube.

Advanced Workflow

100

When resolving system notifications, tap the activated ________ to display information for resolving the problem.

When the analyzer detects inadequate resources or a processing incident, an icon in the Warning Area on the left hand side of software screens will be activated (lit up) to notify the operator of the problem. Tapping the ICON will display the STATUS screen and provide additional information to resolve the problem.

200

STAT status can be assigned to samples either ______ or ______ being placed into the analyzer. 

STAT status can be assigned to samples either BEFORE or AFTER being placed into the analyzer.

300

When crossmatching plasma/serum samples from a patient with donor red cell samples, the ______

and ______ screens display each assigned donor with the icon.

When crossmatching plasma/serum samples from a patient with donor red cell samples, the WORKSHEET

and SAMPLE screens display each assigned donor with theicon.

400

(True or False) If notifications are not resolved within a reasonable amount of time they can interrupt  processing.

True. 

If these notifications are not resolved within a reasonable amount of time, they can become high-level incidents and can interrupt processing. When processing is interrupted, the worksheet fields for those particular test profiles and samples change to grey.

Warnings usually appear only when the system software detects a need for a resource or component to complete processing.

500

List two locations to tap on the touch screen when silencing an audible alarm on the analyzer.

When there is an error on the Eflexis Analyzer, the Alarm icon is activated on software screens. An audio alarm also sounds on the system to alert the nearest operator.

1. Tap the Alarm Icon

2. Anywhere within the Error Message

600

Which is true about loading double sample tubes onto the analyzer?

Check all that apply.

a. Both tubes with the same barcode should be placed in consecutive positions.

b. The analyzer does not need to be configured to accept double sample tubes.

c. The plasma/serum sample tube should be placed behind the red cell sample tube.

d. Both sample tubes are displayed separately on the worksheet.

A. Both Tubes with the Same Barcode Should be Placed In Consecutive Positions. 

D. Both Sample Tubes are Displayed Separately On The Worksheet

700

Describe 7 of these Warning Errors and Give Examples of When these Warning Labels Appear: 

QC

100

True or Fales: Quality Control (QC) samples have expected results for tests that require regularly scheduled QC.

True: Quality Control (QC) samples have expected results for tests that require regularly scheduled QC.

200

If the _______ of QC PASS option has been configured, QC Pass results will automatically be validated.

If the "ENABLE AUTOVALIDATION" of QC PASS option has been configured, QC Pass results will automatically be validated. 

300

Failed QC results are not validated nor are they exported but rather need to be _____ . 

Failed QC results are not validated nor are they exported but rather need to be REJECTED.

400

When QC samples and routine samples are onboard the analyzer, which of the following is NOT TRUE if QC is due for a profile or profiles?

Choose all that apply.

a. Profiles for routine samples may be selected on the worksheet.

b. QC samples are automatically processed before routine samples.

c. Routine samples are stopped and will resume once QC tests are validated with a passing result.

d. Samples that are assigned STAT status will always be processed with priority without waiting to obtain a QC result.

When QC samples and routine samples are onboard the analyzer, which of the following is NOT TRUE if QC is due for a profile or profiles?

Choose all that apply.

(a.) Profiles for routine samples may be selected on the worksheet.

b. QC samples are automatically processed before routine samples.

c. Routine samples are stopped and will resume once QC tests are validated with a passing result.

d. Samples that are assigned STAT status will always be processed with priority without waiting to obtain a QC result.

500

Which eye icon is presented on the RESULTS LIST screen for processed routine samples that require processed QC results to be exported before routine results can be validated?

Which eye icon is presented on the RESULTS LIST screen for processed routine samples that require processed QC results to be exported before routine results can be validated?

Red Eye Icon

600

Routine sample processing is affected by pending QC results. A QC pass result will _____ routine sample processing and change the sample status from ______ to _______.

Routine sample processing is affected by pending QC results. A QC pass result will RESUME routine sample processing and change the sample status from   PENDING VALIDATION to PASS   .

700

Maintenance

100

When working ______ with the analyzer it is recommended to restart it every ______ days to maintain the system software and keep it working correctly.

When working CONTINUOUSLY with the analyzer it is recommended to restart it every 7 days to maintain the system software and keep it working correctly.

200

All of The Above!

Complete a Daily Check for... 

-Leaks or spills in or around the Dilution Station.

-Leaks or spills in the Sample and Reagent Stations.

-Microbial growth on the wash and waste solution containers and lids.

300

True or False: Preventative Maintenance is Performed every 6 Months. 

False: PM is performed Annually. 

400

TRUE OR FALSE: 

Monthly decontamination of the Fluidic System automatically includes decontaminating the Probe as the Dilution Station. The decontamination of the Fluidic System is automatically documented by the system.

However, Probe and Dilution Station decontamination must be documented manually using the Eflexis software.

True. 

Monthly decontamination of the Fluidic System automatically includes decontaminating the Probe as the Dilution Station. The decontamination of the Fluidic System is automatically documented by the system.

However, Probe and Dilution Station decontamination must be documented manually using the Eflexis software.

500

True or False: Damaged, bent, or occluded probe can be replaced without the need for Grifols Technical Service personnel.

True. Damaged, bent, or occluded probe can be replaced without the need for Grifols Technical Service personnel.

600

Before any preventative and/or corrective maintenance is performed by Grifols Technical Service personnel, all __________ and _________should be ________from the analyzer.

Before any preventative and/or corrective maintenance is performed by Grifols Technical Service personnel, all SAMPLES and REAGENTS should be REMOVED from the analyzer.

700

a. Probe

b. Incubators

c. Centrifuges

d. Fluidics

e. Card Transport arm