H&E Staining

Where dye come from?

100

What is the most common staining used in the Histology lab?

Hematoxylin and Eosin staining (H&E)

200

What are the four natural dyes used in histology staining?

Carmine, orcein, saffron, hematoxylin

300

I come from the heartwood of the logwood tree

Hematoxylin

400

I am a purified extract of the female tropical cochineal insect

Carmine

500

I come from the pistils of a flowering plant

Saffron

Making a stain

100

What are the two types of staining techniques used in the Histology lab?

Regressive and progressive staining

200

H&E staining is this type of staining technique

Regressive

300

Eosin can stain 3 different shades of pink. Erythrocytes, cytoplasm of muscle and this

Collagen

400

I am a substance or metal that can act as a link between dye and tissue

Mordant

500

Celestine blue is the most common, but safranin, nuclear-fast red, methylene blue, thionin and toludine blue O are all considered this

Hematoxylin substitutes

Random Bag o' questions

100

I am the most common counterstain when it comes to routine staining in the Histology lab

Eosin

200

Cells can be divided into 2 major parts: the cytoplasm and this

Nucleus

300

This oxidation product of hematoxylin is the true dye we use in H&E staining

Hematein

400

A lake is a combination of mordant and dye. Hematoxylin lakes are combinations of hematein with what two mordants?

Aluminum and Iron

500

A fat stain is an example of this stain 

Physical staining-the dye is purely absorbed by the lipids

Affection

100

This factor in a solution can determine if a dye bind to different tissue elements

pH

200

Eosin should be changed this often

Weekly

300

Water left in the tissue, incomplete drying or inadequate time in xylene can result in this

Incomplete deparaffinization

400

Hematoxylin can develop a metallic sheen of oxidized dye that can cause this if not filtered regular

A blue-black precipitate

500

The change in pH induced by this changes the solubility of the dye lake after nuclear staining

Bluing

Help me out

100

This staining result occurs if sections are cut too thick, left too long in hematoxylin or differentiation is too short

Dark nuclear staining

200

If xylene starts to appear hazy or milky, this indicates that the slides have not been properly what

dehydrated- water is still present on the slides

300

Pale cytoplasmic staining can result from too thin sections or this

Leaving too long in dehydrating solutions

400

If 3 shades of eosin are not distinguishable, it could be because this didn't happen

Differentiation-good dehydration and clearing

500

If hematoxylin was not blued sufficiently or the hematoxylin starts to break down this can happen to the nuclei

It can appear red-brown