Hemoglobin
The Heme portion of hemoglobin is composed of what ?
Protoporphyrin and iron
The most common Leukemia seen in children
ALL
Which type of WBC is seen increased in parasitic infection, increased in allergic reactions and we see 0-4% in normal peripheral blood smear?
Eosinophil
What hormone is responsible for RBC production and where is it produced?
Erythropoietin , kidney
On a cell's surface , specific protein that distinguish one cell line from another is called a CD marker .. CD stands for :
Cluster of differentiation
Which hemoglobin is made of 2 alpha chains and 2 gamma chains ?
Hgb F
The PML/RAR gene is associated with which Leukemia ?
Promyelocytic ( M3)
As WBC matures what happens to the ratio of nucleus to Cytoplasm?
Decreases
What RBC morphology would you see in Pernicious Anemia ?
Macrocytosis
TdT is a marker found on what type of Blast?
Lymphoblast
In order for hemoglobin to combine with oxygen iron must be in what states ?
Ferrous state Fe2+
The philadelphia chromosome present in most cases of CML arises from a translocation of chromosome 9 and 22 which results in what gene mutation that causes unregulated tyrosine kinase activity ?
BCR/ABL
Which WBC phagocytizes microorganisms , is a precursor to macrophages and is increased in bacterial infections ?
Monocyte
What test is used to monitor Red cell production?
Reticulocyte count
What type of cell is CD3+ , CD4+ and Surface Ig Negative ?
T cell- specifically T helper cell
Which hemoglobin has the highest affinity for oxygen ?
A, F, S or C
F
TRAP stain positivity is characteristic of what Leukemia ?
Hairy cell
if the original WBC count is 5000/ul and the tech counts 10 nRBC per 50 WBC what is the corrected WBC ?
4166/ul
Which RBC parameter indicates the degree of anisocytosis?
RDW
Flow cytometry of bone marrow aspirate shows cells that are CD41+, CD42b + and CD61+ what diagnosis does this point toward?
Megakaryocytic Leukemia
how does an increase in Hydrogen ions affect the hemoglobin oxygen dissociation curve ?
Shift to left or right?
Decreased or increased oxygen affinity ?
decreased or increased oxygen release to tissues ?
Right , decreased O2 affinity , increased O2 release to tissue
Which chromosomal abnormality is associated with acute megakaryoblastic leukemia
Down syndrome
What is the cell seen in figure 1 and where does it originate from ?
Plasma cell - B lymphocytes
f you count 75 RBC in the 5 small RBC squares on a hemocytometer after a 1:10 dilution . What is the reported RBC count ?
37,500/ul
A cytochemical test is done on a specimen with the following cell morphology :
round to oval nucleus , cytoplasm is gray with numerous fine projections
CD103, CD11c and CD 25 positive
What type of disease is most likely considered?
Hairy cell leukemia