General Heme Principles
In The Lab
Clinically Relevant
Comparative Species
Blood Typing
100
What 5 things make up plasma proteins?
Albumin; Globulins (including transferrin, coag proteins and fibrinogen).
100
What is the most frequently used anticoagulant for hematological purposes?
EDTA
100
Is the absolute leukocyte count of the % of each more clinically relevant/used?
Absolute count.
100
What species are we dealing with if our blood smear nucleated RBC?
Nonmammalian – e.g. bird
100
When is it safe to use a universal donor for a transfusion in dogs and cats?
Never in cats – there is no universal donor. In dogs you can use any dog once. But will develop antibodies against that type if different from own so can’t do that twice.
200
What 2 things in the body inhibits coagulation in order to keep blood in its liquid form?
Ca2+; Thrombin
200
What is the common used anticoagulant for transfusion purposes?
Citrate
200
What clinical situation might MHC detect?
Iron deficiency at an early stage.
200
In which species is Rouleaux normal?
Horse (and to some degree cats)
200
Most important blood groups in the horse? Why?
A,C and Q – they all have factor alpha and this is important for hemolytic reaction neonatal isoerythrolysis.
300
What is the site of Hematopoiesis after birth?
In the bone marrow.
300
What part in the blood collecting tube is plasma?
The clear liquid at that settles at the top
300
What is suspected if marked anemia but protein normal?
Regenerative anemia due to hemolysis
300
Which species is it normal to see poikilocytosis in the blood smear and what is that?
Abnormal shaped RBC in a blood smear of a goats.
300
What is the best way to choose a blood donor for a horse since they have so many phenotypes?
Pick within its own breed. More likely to find a match
400
What is the color of bone marrow in young vs adult animals?
Young: red. Adult: yellow due to fat replacement.
400
What is the platelet/leukocytes layer called in a blood collecting tube?
The buffy coat
400
What are we thinking if we see the following in a RBC smear: polychromatophils, macrocytic, hypochromic, basophilic stippling and maybe Howell-Jolly bodies?
Regenerative Anemia
400
What might Rouleaux in a dog suggest?
Hyperglobulinanemia (inflammation)
400
How do cats differ from dogs when it comes to blood typing?
Cats have naturally occurring antibodies against blood type antigens that they lack!
500
4 Sites of "Extrameduallary hematopoiesis"?
Spleen, Liver, Lymph nodes, Kidney
500
Which anticoagulant works by inhibiting antithrombin III and why would this be used?
Heparin. Used if you want to analyze the plasma.
500
What is regenerative anemia often concurrent with?
An inflammatory leukogram
500
Where is hematopoiesis in reptiles, amphibians and fish?
Reptiles: Bone marrow and spleen Amphibians and fish: spleen, kidney and liver
500
What may occur if a type AA Tom is bred to a Type bb queen?
The kitten will be Type A and will be at risk when it nurses on the moms milk which will contains naturally occurring antibodies to Type A antigen – so those will destroy the Type A on the kittens RBCs.
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