C. elegans
What are C. elegans classified as?
nematode/roundworm OR model organism
Name the 7 steps of cloning Plasmid 1
1. PCR
2. Gel electrophoresis
3. KLD Ligation
4. Transformation
5. Colony Selection
6. Miniprep
7. Sequencing (+ Archiving)
What is the name of the lab mascot?
Elle elegans
Name the scientists the Houses are named after (first and last name)?
Sydney Brenner, Martin Chalfie, Rosalind Franklin, Jennifer Doudna
What is the maximum amount the red micropipette can pipette?
2 uL
What keeps worms "worm-shaped"?
the cuticle
Who is known for pioneering work in CRISPR gene editing?
What happens if you don't wear pants to lab?
OU Pants of Shame + Wall of Shame
What were the names of the structures of wet lab before and after Spring Break?
Guided Lab and Open Lab
What is the website we use to view the C. elegans genome?
Wormbase
What is the approximate life cycle of C. elegans?
3 days
What phenomenon organizes cells?
Cell polarity
What is the most viewed reel on @utglowworms?
Gel Talk with Glow Worms
Who gives us money to run the stream?
Dr. Daniel Dickinson 🤑
What is the name of the tool used to pick colonies (spell it!)?
Inoculation Loop
What is the Dauer stage?
starved worms/hibernation due to adverse conditions
Name the different stages of the C. elegans lifecycle
Embryo, L1, L2, L3, L4, Adult
What is Naomi's cat's name?
Birdie
What company does sequencing for us?
Eton Bioscience
What is the purpose of KLD Ligation?
How many neurons are in the C. elegans nervous system?
302
What are the enzyme(s) involved in KLD Ligation?
KLD = Kinase-Ligase-Dpn1
Name 10 mentors.*
mentors can confirm if correct :)
What is our lab room number?
WEL 3.302
How long is the annealing stage of PCR (on thermal cycler)?
10 seconds