Quantikine
Validated sample types for Qkits
What is - Serum, plasma, cell culture supernates (not all kits, milk, urine, saliva, tissue lysates, and tissue homogenates)
Components within Duoset kit
What is~ Capture, Detection, Standard, and Streptavidin HRP
What is~ up to 50
Number of Membranes within an Array kit
What is ~ 4 membranes per kit (some have 8)
Kits that can be run as a partial plate
What are~ Quantikine and Duoset ~ Quantikine comes with removable strips, Duosets depend on the plates they purchase, but can be run partially. Luminex has black plates that can be covered for partial runs.
Number of samples for Qkits
What is ~ 40
Most Quantikine ELISA Kits will run the standard curve and 40 samples in duplicate.
Validated sample types for Duoset
Definition of Multiplexing
What is ~ multiplexing allows multiple biological target analytes to be simultaneously examined and quantified in a single sample. It’s a technique widely used by scientific researchers of all disciplines and provides many benefits.
Validated sample types for Array kit
What is ~ Serum, plasma, tissue lysate, cell lysates, (some breast milk, urine, saliva, tissue homogenates, etc)
Explain the mechanism of sandwich ELISA
What is ~ A sandwich ELISA uses an immobilized capture antibody specific for the analyte of interest in a sample. After the analyte is bound to the immobilized antibody, a labeled secondary antibody specific for the analyte is used for detection. The analyte is "sandwiched" between the two antibodies
What is the master calibrator?
What is~ Test against our master calibrator for consistent immunoreactivity.
Importance of Wavelength Correction
What is~ This subtraction will correct for optical imperfections or refraction created by the plastic plate
Describe split based dilution
What is~ This keeps analytes requiring the same dilution factor within a single panel, while separating analytes requiring different dilutions.
Number of samples used to analyze in Array kit
What is ~ 4 samples per kit
Explain the mechanism of Competitive ELISA
What is ~ In the competitive immunoassay approach, also termed "labeled analyte technique", there exists a competition between the endogenous unlabeled antigen and an exogenous labeled antigen for a limited amount of antibody binding sites. Therefore, a decreasing signal indicates higher concentrations of the analyte being measured.
Expiration for Qkits
What is~ dependent on the component with the shortest shelf life. But a minimum of 12 weeks (shipdown date)
Controls for Duoset kit
What is~ QC control sets are not available for DuoSet ELISAs as they are developmental assays with only core reagents- End-user will need to find their own
Software used to analyze Luminex results
What is ~ Quantist (xPonent can also analyze)
Normalization uses within an Array kit
What is ~ You cannot normalize an Array kit as it is a Qualitative assay.
Array kits are designed to compare relative expression levels of analytes in treated and non-treated samples. Reference spots consist of an unrelated biotinylated protein spotted directly to the membranes.
Signal from these reference spots indicates that the kit detection system is working and also provides guidance spots for aligning the membrane to the template
Elispot uses/application purpose
What is~ used for detection of cytokine-secreting cells
Customer received Qkit and needs more wash buffer
What is ~ Wash buffer can be purchased separately.
Wash Buffer Concentrate, 25X Catalog #: WA126
Difference between Quantikine and Duoset kit
What is~ Duoset kit only has 4 components and will need ancillary materials purchased by end-user. User coats plate
Quantikine kit has most of the necessary materials to run kit. Pre-coated plates.
Difference between discovery and high performance kit
What is~ A High Performance Assay consists of select analytes which have been thoroughly tested and validated together as a panel. The diluents provided in the High Performance kits and assay conditions have been optimized for best results with the analytes on the same panel. The High Performance Assay is the most precise, reproducible and accurate Luminex tool offered by R&D Systems and are correlated to our gold standard Quantikine ELISA kits. Controls are available for the Human Luminex High Performance Assays. The Luminex Discovery Assay option is a flexible tool, providing the uncommon opportunity to customize by mixing and matching analytes across different panels. Although not as extensively tested as the High Performance Assays, analytes on a Luminex Assay are tested together when built and a final quality control test on each Luminex Assay is performed prior to release. Since each Luminex Assay is unique and built to order, additional days are required for order fulfillment and it is not characterized by the same level of optimization and validation testing as with the High Performance assays. Controls are not available for Luminex Assay. In general, the sensitives of the Luminex Discovery Assays and Luminex High Performance Assays are similar. For low abundance cytokines, High Sensitivity panels are available for High Performance Assay, which are not available for Luminex Assay.
Detection system used for Array kits
What are~ film, camera, (Some kits have been validated for Li-Cor)
Define Matrix Effect
What is~