Infectious Disease Serology
What is the reference range for the prenatal Rubella antibody test?
Positive
Serum C3 levels would be __________ in a patient with active SLE?
Decreased
The ANA test we performed in lab was a (direct or indirect) method?
Indirect
Which antibody class is produced first during an immune response?
IgM
What dilution is made when 2mL of serum is added to 8mL of saline?
1:5
Which test is routinely used to screen for syphilis?
RPR
Acquired immunodeficiency syndrome is caused by:
HIV
A homogeneous pattern on the ANA test is strongly associated with:
SLE
Which antibody class is involved in allergic reactions?
IgE
A test for virus antibodies is negative. The package insert states that all negative serum specimens must be retested with a 1:10 dilution. This is intended to prevent false negative results due to:
Which test can be used to rapidly diagnose acute EBV infection?
Monospot (Rapid Mono test)
IgG
The type of agglutination method wherein the antigen is coated on the surface of the carrier particle.
Passive agglutination
Which antibody class can cross the placenta?
IgG
You setup a serial dilution in five tubes with neat serum in tube #1 and 1:2 dilutions in tubes #2-#5. What is the solution dilution for tube #4?
1:8
Which test can be used to diagnose acute rheumatic fever?
ASO
CRP rapidly increases during infections. This classifies CRP as an ________ ________ ________.
Acute phase reactant (protein)
What method did we use in lab to measure C3 & C4 levels?
Precipitation (radial immunodiffusion)
Which antibody class can be found in saliva, tears, and breast milk?
IgA
An RPR titer is run on a reactive serum specimen. Circle #1 is neat and the serum was serially diluted 1:2 in circles 2-8. Circles 1-7 are reactive. What is the titer?
1:64
Name the four infectious agents tested for with the ToRCH panel.
Toxoplasma gondii, Rubella virus, CMV, HSV 1 & 2
Centromere
Name a confirmatory test for syphilis.
FTA-ABS or TP-PA
Heterophile
An aliquot of a 40% solution of RBCs was placed in well #1 of a microtiter plate and serially diluted (1:2) in wells 2-5. What is the percent (%) solution of RBCs in well #4?
5%