Lab Safety
Chemical spill kit is located where?
Lab 5-2 under the sink
Where are SOPs stored?
In Lab 5-2 in the cabinet and in SharePoint under Quality Systems (for now), and MediaLab (new).
Cloth shoes are allowed in the lab.
No, cloth shoes can absorb chemicals and hold them against the skin until they can be removed.Shoes need to protect the wearer. No sandals, open-toed or perforated shoes.
What should you do if you splash ethanol in your eye?
Immediately go to the eye wash, DO NOT remove contacts if wearing, have a co-worker hold your eyes open to rinse them for 15min. Notify manager/supervisor to fill out incident report. Seek and get medical attention.
Delay caused in removing contacts could cause injury.
Driftcon verifies what in the lab?
96 wells for the thermocyclers
If you have long hair, how should you come to the lab?
Long hair should be tied back and out of the way
Pipette calibration schedule
every 6 months, June and December
Personal electronic devices, such as phones, are able to be used in the lab.
False, use of personal electronic devices in the lab is not recommended in the technical work spaces due to the potential for biohazard or chemical contamination, distraction, and intellectual property violations.
Steps for operating a fire extinguisher
PASS
Pull
Aim
Squeeze
Sweep
The inspector asks for a list of individuals that can use the array scanners. Refer them to this log.
The Equipment User Log
What should the biohazard carboys have on them?
Label defining what is contained in the container, and if flammable, a flammable sticker. Labs are lab room specific and will be green if non-flammable, and red if flammable. Start date with initials should be on the label.
You're at work the day after a holiday when work was closed. How do you take temperatures?
For the holiday, follow the form to see what to write when not taken/in use (e.g. X or n/a) then date and initial and write 'Holiday'. Take min/max temperature. Remember the Picopure logs are different on Holiday days.
Aerosol barrier tips are used to prevent contamination
True
How do you remove PPE?
1. Remove Gloves
2. Remove Eye Protection
3. Remove Lab Coat
SDS stands for what and where is it located
Safety Data Sheets are located on SharePoint under Quality Systems
Ergonomics Plan
what logs are in the lab for cleaning?
Weekly Cleaning
Daily Decontamination
When Pre-PCR is transferred to POST-PCR the following is done: PCR plate is transferred to POST lab bench wearing white coat and gloves making sure not to contaminate PPE. Without touching anything in POST, Go back into Pre and remove/doff. Go back into Post and don post coat and gloves.
True
Reagent validations must be performed when received and during discovery of freezer/fridge temperatures outside of this criteria.
if temp is out of range ≤ 5°C for more than 2 hours, or if temp is out of range more than 5°C for 1 hour
What is recorded on Maintenance logs?
software errors, instrument errors, scheduled and unscheduled maintenance.
What does RACE stand for and when is it used?
Used during fire drill and if actual fire/emergency evacuation.
RESCUE
ALARM
CONTAIN
EXTINGUISH/EVACUATE
Before and after dumping biohazard waste, what should be checked?
proper labels on the carboys, the cap is put back after use (screwed on), bin containing the carboy is free from paper towels/gloves/trash, etc.
HIPAA's most severe breach can result in up to 10 years in prison and a $250,000 fine
True. Violation in accessing and using PHI are subject to HIPAA's laws. Minimum necessary rule: use the least amount of PHI needed to get the job done.
What does HIPAA stand for?
Bonus of 100 points if you correctly answer what year.
Health Insurance Portability and Accountability Act. It's a federal law designed to protect a subset of sensitive information known as PHI (protected health information).
1996
Instruments verified for comparability testing are what and when?
ABIs, Nanodrop, Qubit, Scanners, QuantStudios. Every 6 months, April and October