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What is Bacteriology?
A bacteria normally found in the intestinal tract - genus and species.
E. coli
What color are Gram negative bacteria?
pink
How many BSL are there?
4
controls the growth of organisms on living tissue
Antiseptic
What is the best type of collection swab?
Dacron
Which type of media is a solid material that fills a shallow plastic dish with a loose fitting lid?
Agar
Microbes that cause disease
Pathogen
A bacteria normally found on the skin
Staphylococcus epidermidis
Which part of the cell wall is responsible for shape and staining?
peptidoglycan
Which BSL is the most hazardous?
BSL4
Reduces the number of organisms to a level that is no longer a health threat
disinfectant
What the term for media that contains a population of organisms?
inoculum
What is agar poured in tubes and allowed to cool at an angle?
The study of fungi.
Mycology
What are the 3 main purposes of clinical microbiology?
1. Isolate
2. Identify
3. Antibiotic Susceptibility
What is the classification system/ orderly method for placing microbes into categories?
taxonomy
Which BSL contains the most often identified organisms in routine microbiology samples?
BSL 2
What conditions must be met to sterilize using an autoclave?
121oC for 15 minutes at 15 psi
What is used in the lab to prevent lab acquired air borne infections?
Safety cabinet or hood with a HEPA filter
Which type of media is a clear liquid when sterile, but cloudy when bacterial growth is present?
Broth
Does not usually cause disease in a healthy person
Opportunistic pathogen
What term is used to describe microbes that produce disease?
pathogenic microorganisms
What bacterial special feature allows resistance to heat, drying, cold, or chemicals?
sporulation
Which BSL contains Mycobacterium tuberculosis?
BSL3
What is the most common antiseptic?
70% Alcohol
Lab results are only as good as the _________.
specimen
Which term describes bacteria that cause disease only when normal defense mechanisms are impaired?
opportunistic pathogen
Infections acquired in the hospital
nosocomial or iatrogenic
What is a body site or fluid that SHOULD be sterile?
blood, spinal fluid, urine
What 3 criteria are used to categorize bacteria?
morphology, gram stain, biochemical reactions
Name 1 bacteria in BSL 2.
Salmonella
Shigella
many more........
What is the process of transferring a population of organisms to a growth medium?
Inoculation
What can be used to preserve bacteria before it reaches the lab, yet inhibit grown?
Transport Media
What is the term for rod shaped bacteria?
Bacilli
(round is cocci)