Is there 'ANTIBODY' out there?
To report an allo-antibody without comments or additional wording, what is the minimum requirements?
Reactivity with at least two antigen-positive RBCs
Non-reactivity with at least two antigen-negative RBCs.
Patient phenotype/genotype negative.
When does an AUTO control need to be tested?
Each time the patient is submitted with initial antibody testing.
If you suspect rouleaux in the forward type of your ABO, because of other observations or diagnosis, what should be done?
Look for rouleaux and repeat washing cell suspension 3 times.
This elution procedure uses temperature changes to elute antibodies to the ABO system?
Lui Freeze-Thaw
What does "slay" mean?
Something described as very good or amazing. Usually used with YASSSSSS Ex: YASSSSS! My outfit is SLAYING today!!!!!!!!!(dramatic hair toss)
If double-dose RBCs are not available for exclusions, what methodologies may be used to exclude common antibodies?
Polyethylene glycol (PEG)
Solid phase
Enzymes for antibodies to antigens reported to be resistant to enzyme treatment
Gel
How long does the patient's last transfusion have to be before Auto Adsorptions can be performed?
3 months
What is the control used for 0.2M DTT treatment of cells?
A reagent red cell with a double-dose expression of an antigen from the KEL system and the corresponding antisera.
If you are testing the Eluate to investigate anti-A,B, what reagent RBCs are tested?
At least 2 A1 RBCs, 2 B RBCs and group
O cells sufficient to rule out common alloantibodies.
If you have rizz, you are?
You are charming.
If a patient has anti-C and anti-E, what special rule do you need to remember for antibody exclusion?
Test with at least three D+C-E- reagent RBCs to rule out anti-D.
What does it mean if you identify an antibody with specificity, but the corresponding antigen typing is positive?
Could be an auto antibody or a variant antigen expression.
If an IGM antibody is reacting at 37C or IAT, how do you determine if it is clinically significant?
Perform prewarm settle.
BONUS if you can give the steps!
When is an Elution required? (2)
1. When there is a positive DAT and the patient has been transfused within the past 3 weeks.
2. Required on all 1st time WAA patients.
What challenge became popular in 2022 where participants play Rock, Paper, Scissors with a mouth full of water?
Tortilla Challenge
When expired reagent cells are used, what are the 3 manufactures that that do not need further testing?
Ortho, Werfen (Immucor), and Medion Grifols
What common antibodies do not need to be ruled out if adsorbed testing is being used?
Ruling out anti-Lea, -Leb, and -P1 is not required when testing adsorbed sample unless suspected in initial testing.
What does 0.01M DTT treatment of cells do?
Removes any bound IGM antibodies causing complications or unexpected reactivity with controls.
In the Lui-Freeze-Thaw procedure what are the amounts of RBCs and saline added to the elution tube prior to placing in the freezer?
0.5 mL of RBCs and 3 drops of saline.
In "Wicked" what is the name of the school that Galinda and Elphaba attend?
Shiz
In WST, how do you enter historic antibodies in a patient record? Bonus 100pts if you give step by step instructions.
You go to "Antibodies".
Bonus!
1. Patient module.
2. Active Patient screen.
3. Click the patient’s information bar to select.
4. Click “Antibodies.” and Add.
In WST which orderable antibody screen test includes the autocontrol?
SCREEN includes AC, ABSC is without AC.
Is serum or plasma more likely to demonstrate rouleaux?
Plasma
When using Gamma Elu-Kit II modified antiglobulin technique, what is omitted and what is the modified process?
The repeat washing with normal saline is omitted, instead 5-10 drops of Working Wash may be used.
If you had a "brat" summer, you most likely listened to this artists album?
Charli xcx