BMDM
What bone provides the primary source of bone marrow?
What is the femur?
The chromosomal location of TSC22D3?
What is the X chromosome?
What are the ligands for LILRB4?
What are Alcam/CD166, Apolipoprotein E, Fibronectin, and Galectin-8?
What is the transmembrane receptor that binds growth factors and enhances growth and survival of breast cancer
What is “HER2” -or- “human epidermal growth factor receptor 2”
What is the goal of vaccinating mice?
What is to produce antibodies
What is the 'panic button' for macrophages?
What is Lipopolysaccharide (LPS)
What is hypoxia?
Low oxygen saturation, typically found in the tumor microenvironment
What are the three signals to activate T cells?
1) MHC binds to T cell receptor; 2) Costimulatory binding (CD80/CD86); 3) Cytokines are released to promote T cell longevity (e.g. IL-2)
What are our "jumpiest" mouse strains?
What are PWK and WSB?
Name at least two methods of euthanasia in mice
What is (any 2 of the following): CO2 inhalation, barbituate overdose, cardiac puncture, cervical dislocation, bilateral thoracotomy, decapitation, vital tissue harvest
What activation markers do we typically look at on macrophages?
What is CD80, CD86, MHC I, and MHC II?
What reagent is used during transfection to encapsulate DNA?
What is lipofectamine?
What is the full name of LILRB4?
What is leukocyte immunoglobulin-like receptor B4
What is the Neu protein? How does it relate to our work?
What is the rat homolog of the human HER2 protein? Drives spontaneous mammary tumor growth in mice.
Why are transfected cells often plated with antibiotics?
What is to keep the plasmid which provides antibiotic resistance to the cell?
What is the background mouse strain of the LILRB4 knockout?
What is B6?
What does TNBC stand for? What is one cell line that is a TNBC?
What is triple negative breast cancer? D2F2 and 4T1
This protein is a checkpoint molecule on natural killer cells.
What is NKG2a?
Why is using Diversity Outbred mice important?
What is they mimic human diversity
What is a method that we used to measure immune reactivity? What immune mechanisms do they relate to?
What is Elispot (T cell response)? What are sera antibodies measured with flow cytometry (B cells)? What is inhibition of tumor growth (both)?
What does M-CSF stand for and what does it do?
What is macrophage colony stimulating factor - Differentiates bone marrow stem cells into macrophages
What is the purpose of a leucine zipper structure?
What is our current hypothesis on how LILRB4 affect T cell activation?
What is antibody drug conjugate. We use Trastuzumab deruxtecan.
What are our main genetic modifications to our mouse strains? What strains to they belong to?
LILRB4ko (B6 background), d16HER2 (FVB background), NeuT (BALB background)