Mechanics of
Development
Development
True or False:
Cancerous tissue is stiffer than healthy tissue.
True
What protein makes the outer layer of skin tough?
Keratin
What is morphogenesis?
the biological process through which organisms, tissues and cells develop or change shape
True or False:
The drosophila genome is not fully mapped.
False
What two techniques were used in paper 3 to knockdown or edit genes?
CRSIPR-Cas9 and RNA interference
When researching axonal growth, did axons grow farther, straighter, and stiffer on soft or stiff substrate?
Stiffer Substrates
What edge of the cell does Lar localize to in cell movement?
Leading Edge
What is the origination of the word Morphogenesis?
It is greek and means "emergence of shape"
What is an advantage of suing a chicken embryo?
develop in egg making it easy to monitor or abundant and easy to obtain
Why is MPM ideal for live imaging?
Can see deep into tissue with minimal damage
What junctions mechanically couples cells together?
Adherens junction
What are 3 examples of Cell-Cell Junctions?
Desmosomes, Gap junctions, Tight Junctions/separate junctions, Adherens Junctions, Ig-CAMs
What are three applications of morphogenesis?
Understand disease, injury healing, ensure organ structure, cell function and shape, and understand how force changes a cell
What are two disadvantages of using the zebra fish?
Development timing, Simpler eye structure, or Environmental differences
What technique is used to the surface forces produced by a cell?
Traction Force Microscopy
What behavior in drosophila embryo is an example of convergence and extension?
Germband Extension (GBE)
What is the Reaction Diffusion Model?
A mathematical framework that can recapitulate patterns found in nature
What are three drivers of morphogenesis?
What are two things that make drosophila a good model organisms for morphogenesis specifically?
Conserved Pathways, Visible Developmental Stages, Scalability for Genetic Studies
What shape was ideal for measuring forces using intravital mechano-sensory hydrogels?
Cylinder
What are four cell behaviors that shape tissue?
Shape change, rearrangement, delamination, extrusion, division, and integration
What type of adhesions could drive Morphogenesis?
Differential
Who are two people who contributed valuable information on morphogenesis?
D'Arcy Thompson, Hans Driesch, Alan Turing, Conrad Waddington
What were the four common morphogenesis model systems listed?
Fruit fly, zebrafish, mice, and round worm
What does FRET-based tension Sensors measure?
measure mechanical forces by detecting changes in fluorescent intensity