Zymography
It was measured by zymography but could not be measured by Western blotting
Activity of Enzyme
Two Crops that are most often genetically modified
Corn, Soy
Literally meaning “cysts due to thickening and scarring of connective tissue”, the disease we diagnosed by restriction digest
Cystic Fibrosis
Cell type that produces extracellular matrix and collagen – we grew them for protein isolation
Fibroblast
An anti-apoptosis protein, its name stands for “B-cell lymphoma-2”
Bcl2
The type of data that ImageJ produced from a gel picture
Band Intensity/ Quantitative data
The feature of GAPDH making it a good positive control for PCR
Housekeeping gene
The gene encoding a transmembrane protein tested
for mutation
CFTR
Tool for counting the number of cells under a microscope
Hemocytometer
The solution that supports cell division by providing proteins
(like growth factors, enzymes, and hormones)
FBS, Fetal Bovine Serum
Substrate broken down by MMP's in the lab
Gelatin
The chemical used to precipitate DNA from a cellular solution
Isopropyl alcohol/ Isopropanol
Along with EcoRI, the enzyme used to digest DNA
HindIII
Spraying ethanol, a flow hood, and closed bottles – together they support this technique
Sterile/ aseptic technique
A compound that inhibits DNA topoisomerase - it was discovered by its anticancer properties
Camptothecin
Region of Animal Cell represented by Condition Media
Extra Cellular Matrix
Along with the 35S primer, the plant serving as a positive control
Starlink Corn leaf
The number of bands produced if a restriction enzyme is added to a DNA molecule with 4 cut sites
(assume bands of different sizes)
5
A protein used to release adherent cells from the flask
Trypsin
Named for the scientist who first proposed it, this unit compares proteins by atomic mass
Dalton, KiloDalton
Compared to Western blotting, this specific omission in sample preparation is essential for zymography
beta-mercaptoethanol, Heat denaturation
Along with annealing and extension, a step needed during PCR
Denaturation, 94degree Celsius
The two most important band features when analyzing restriction digest patterns
Band Size, Number of bands
The reason trypan blue is added to cells before counting
Identify dead cells
After completing the Western blot, one specific experimental error that could cause this result:
(any one of these)
didn’t extract/load any protein
proteins ran out of well/gel
Bcl-2 degraded
transfer failed
forgot to add:
primary or secondary antibody
chemiluminescent substrate