Enter CategoryGenes So Similar They Must Be (cor)Related!
100
In Illumina sequencing, this modification to a nucleotide allows the addition of each nucleotide fluorophore to be viewed one at a time.
What is a reversible terminator?
100
This enzyme synthesizes DNA using RNA as a template.
What is reverse transcriptase?
100
This type of node has a disproportionately high amount of connectivity.
What is a hub?
100
This type of plot can be used to visualize the quantile distribution of transcripts between different microarray or RNAseq experiments.
What is a boxplot?
100
In performing clustering, the main goal is to maximize this between genes that are grouped within a single cluster
What is similarity?
200
Although this instrument produces longer read length than any other next-generation sequencer, its sequencing reads contain high error rates that can limit its use.
What is Pacific Biosciences?
200
The entire complement of all transcripts produced within an organism including all cell types, tissues and in response to the environment.
What is a transcriptome?
200
This property can only be determined by looking at the system as a whole and not at individual variables.
What is an emergent property?
200
This distribution best approximates RNAseq data.
What is negative binomial?
200
Similarity in gene expression is calculated and determined based on a type of this.
What is a distance metric?
300
Production of clusters with Illumina sequencing uses this process.
What is bridge amplification?
300
A color-scale graphical representation used to represent the levels of many different transcripts across many different treatments or conditions.
What is a heatmap?
300
This network metric describes the proportion of edges exiting a node.
What is out-degree?
300
This property of a transcript is taken into account when normalizing read count within an RNAseq experiment.
What is cDNA length?
300
A similarity measure in which genes with similar expression patterns, rather than expression magnitude, are grouped together.
What is Pearson correlation?
400
This aspect of DNA synthesis is utilized in both Illumina and Pacific Biosciences sequencing and is essential to DNA’s primary structure.
What is "formation of a phosphodiester linkage”?
400
This type of transcriptional processing is taken advantage of when synthesizing cDNA from RNA.
What is addition of a polyA tail?
400
This type of network motif is often used when an entire biosynthetic pathway needs to be activated suddenly.
What is a single-input module?
400
This type of statistical test can be used to detect differential expression between two treatments, but not any more than this.
What is a t-test?
400
A method of clustering in which one begins with a single gene, and successively joins the nearest clusters until all genes fit into a single "super cluster."
What is hierarchical?
500
Unlike others of its kind, this enzyme aids in DNA sequencing by allowing fluorescence to be excited and detected in real time.
What is a “pause” polymerase?
500
This type of molecule can be used to determine the function of a long non-coding RNA.
What is a siRNA?
500
This type of mathematic description can capture the probability of each interaction occurring within a network.
What is Bayesian?
500
Normalization methods are used to ensure similar variation between experiments so that this can be carried out.
What is "detection of differentially expressed genes"?
500
A method in performing data analysis in which there is no specification by the user to identify a certain pattern; rather, the data tells the user which patterns are found. This method occurs with most clustering approaches.