All About Sequencing
Normalization
RNA
Networks
100
This method of sequencing uses chain-terminating dNTP's, which are either radioactively or fluorescently labeled.
What is Sanger sequencing?
100
Both the poisson and negative binomial distribution are classified as this.
What is discrete probability distribution?
100
Made of endogenous genes.
What is mRNA?
100
In a type 1 coherent FFL, the target gene 3 is controlled by this logic. (It requires the product of X and Y gene).
What is an And Gate?
200
The 454 platform uses this method of sequencing.
What is pyrosequencing?
200
The two areas of the graph that split and the lowest and highest 25 percent of the data is examined.
What are the upper and lower quartiles?
200
A single stranded RNA transcript that is destined to become an miRNA becomes double- stranded as a result of this feature.
What is a stem loop structure?
200
The three most common network motifs in biology.
What are FFL, SIM and DOR?
300
This kind of miscall is common in Illumina sequencing.
What is A>T?
300
The best to use to compare between multiple samples.
What is ANOVA?
300
A single stranded RNA that binds to Argonaute protein.
What is a guide strand?
300
Something that can be both a node and an edge in a gene regulation network.
What is a transcription factor?
400
It takes about this long for a paired-end read run on an Illumina HiSeq machine.
What is eight days?
400
This method of cluster analysis aims to divide observations into clusters, with each observation belonging to a cluster to the nearest mean.
What is k means clustering?
400
The location on the chromosome is where you can find most of the small RNA complimentary sequences.
What is a centromere?
400
A network structure common in development related genes, because it allows the sequential activation of a group of genes by a single input signal.
What is a Single Input Model?
500
In pyrosequencing, this enzyme is used to degrade dNTPs after a reaction is completed.
What is apyrase?
500
One of these distance metrics looks at the level or magnitude of the data, the other looks at the size or shape, but both are used primarily for determining this.
What is distance covariance?
500
In order to determine the sequence of cleaved mRNAs in a RNA-seq deep sequencing study, use adapters to recognize this structure and also moiety when constructing a cDNA library.
What are poly-aaa and 5- methylphospate/5mp?
500
X activates Y. Y inhibits Z. X activates Z. A drug that activates Y to cure a disease caused by the lack of X. (X does not act with anything else other than Y or Z).
What is bad drug?
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