Fundamental Skills
This technique reduces the amount of contamination when working with microbes.
What is aseptic?
These are tubes with agar at an incline.
What are slants?
This is a method for streaking an agar plate that uses 4 streaks.
What is a quadrant streak?
This is the special paper that is used to clean the lenses.
What is lens paper?
This stain uses only a single color to create contrast.
What is a simple stain?
The number of viable microbial cells in a sample that can multiply to form a colony.
What is colony-forming unit?
These are colony shape, margin, elevation, pigment, and texture.
What are the categories of colony morphology?
This is the concept that microorganisms are found everywhere on Earth.
What is ubiquity?
The objective lens used to begin looking at a specimen.
What is the scanning objective?
This is a stain that colors the organism but not the background.
What is a positive stain?
This method reduces the amount of contamination when opening test tubes.
What is the purpose of flaming around the opening of a test tube?
Color and growth pattern morphologies.
What are the two categories of morphology for bacteria grown on slants?
This references only the edge of the entire colony
What is margin morphologies?
The purpose of oil when using this lens is to allow more light to enter the lens and increase magnification.
What is the oil immersion lens?
This method fixes the bacteria to the slide for staining.
What is heat fixing?
The purpose of this method is to isolate individual colonies using reduced amounts of cell density.
What is streak plating?
This type of broth growth pattern results in suspended chunks floating in the broth.
Types of pigments in colony morphology.
What is the color, translucent or opaque, and shiny or dull?
This gets smaller as magnification increases.
What is field of view?
This type of bacterial cell will turn purple in Gram staining.
What is Gram-positive?
This is labelling test tubes with your name, date, and the name of the organism.
What is the first step in aseptic technique?
This pattern in broth has a mass at the bottom of the tube.
What is a sediment growth pattern?
This growth pattern on slants has a crusty appearance.
What is a friable growth pattern?
This concept was utilized when looking at colored threads stacked together.
What is the depth of field?
This solution creates crystal violet-iodine complexes.
What is the iodine solution?