Protein Quantification
What does the Lambert-Beer Law state?
increase absorbance = increase concentration
LINEAR relationship between absorbance and concentration of substance in a solution
What wavelength is guaiacol absorbed at?
500nm
Do endothermic or exothermic animals regular their own body temperature?
Endothermic!
Why do the leaf disks float?
they are producing oxygen
Fill in the blank:
_______ is a type of KE used to describe all different kinds of energies released into space by the sun.
Electromagnetic radiation
How does enzyme concentration affect rate of reaction?
The rate of a chemical reaction increases as the substrate concentration increases. Enzymes can greatly speed up the rate of a reaction. However, enzymes become saturated when the substrate concentration is high.
How do you calculate the metabolic rate?
5 mL O2 times Temp = Average time (min)
What's the equation for photosynthesis?
6H2O + 6CO2 + Light -> C6H12O6 +6O2
What does a short and long wavelength translate to in terms of energy?
Shorter = Higher energy
Longer = Lower energy
How might differing pH values influence the differences in the rate of enzyme activity?
Think about what can happen to an enzyme when you change its pH
The protein structure of the enzyme is changing shape as the pH changes
What protein do free fatty acids turn on and where in the cell does it do it at?
Thermogenin
Mitochondria
Why do we need the rate of respiration to find the rate of photosynthesis in the light?
to find the rate at which carbon is getting incorporated into the plants tissues
What is the purpose to the blank?
Blank will help serve as control and calibrate machine so any background abs of the solvent and cuvette will be zeroed out.
What is peroxidase's substrates? When these substrates are reacted together, what are the products?
1. Substrates: Hydrogen peroxide and guaiacol
2. Reaction: 4H2O2 + 4C7H8O2 -->8H2O + C28H21O8
hydrogen peroxide + guaiacol ->water + tetraguaiacol
Protons go through ATP synthase and make ATP
When we are cold, protons go through the UCP (uncoupling protein) and converts it into ONLY heat and not ATP
In the Light Absorption Characteristics of Chlorophyll part of the lab, absorbance and wavelength colors were measured as different wavelengths. As you increase wavelength, the color changes. Why?
the colors we see are produced when light of a particular wavelength hits the right photoreceptor cells. Blue light is from short wavelegths and red is from longer wavelengths
What conclusion can you make about peroxidase's optimal environment?
Enzyme activity increases as the temperature increases
Too high temp can denature protein
22 degrees Celsius is optimal temp
optimal pH = 4.0
List at least 3 enzyme characteristics.
1. not used up in reaction
2. can be reused
3. shape is important for binding substrate @ active site
4. enzymes prefer a specific environment
5. can be denatured
What is the IV and DV? State your conclusion?
DV: rate of respiration
Why do you think we are using the time it takes half of the disks to float as our estimate of the rate of photosynthesis or respiration?
the disks float when bubbles are made. The bubbles contain oxygen which is a by product of photosynthesis so more bubbles=more photosynthess