Lab Modules
1-3
1-3
This type of microscope produces high resolution images of specimens as small as nanometers in diameter. They can magnify objects up to 2 million times.
What is electron microscopy?
The name of the culture that contains only one species of microorganism
What is a pure culture?
Streptococcus pneumoniae contains capsules and causes which disease?
What is classic bacterial pneumonia?
This type of media contains a variety of nutrients that will facilitate the growth of a wide range of microbes. Examples include TSA
What is General-purpose or all-purpose media?
This type of microscope is used during lab
What is light of brightfield microscope?
This part of the microscope is used to focus the light going through the microscope
What is condenser?
This type of media allows for distinctions to be made between similar organisms by targeting specific biochemical properties of microorganisms.
What is differential media?
This is the primary stain in the capsule stain
What is % aqueous crystal violet?
The distance between the slide and the objective lens
What is working distance?
This type of stain stains the background and leaves the bacteria unstained, improving the contrast between cells and background
What is negative stain?
The ability to see two separate objects as discrete entities
What is resolution?
The names of the two commonly used negative stains (dyes)
What is nigrosin and India Ink.
This is the counterstain in the acid-fast stain
What is methylene blue
This acts as the decolorizer in the capsule stain
What is copper sulfate?
The primary stain in the endospore stain
What is malachite green?
This type of microscope has a modified condenser. It is used to enhance the contrast of transparent or colorless specimens
What is phase contrast microscopy?
The two types of simple stains
What is negative stains and positive stains?
A negative result for motility using a TTC (triphenyltetrazolium chloride). A stab inoculation appears as:
What is a solid red stripe at the stab line only?
Species such as Bacillus anthracis, Clostidium tetani and C. botulinum have which bacterial structure?
What is endospore?
This acts as the mordant in the acid-fast stain
What is steam (heat)?
The function of oil immersion
What is decrease the loss of transmitted light, and increases image clarity (resolution)
Two examples of dyes that are commonly used in positive stains
What is Crystal Violet and methylene blue?
Non-motile organisms appear to vibrate or shake caused by the collision of the cells with water molecules due to kinetic energy. This is known as:
What is Brownian motion?
The way that mobile microorganisms move under a microscope is known as:
What is microorganisms move in various directions, in random runs and tumbles?
A positive result for motility using a TTC (triphenyltetrazolium chloride). A stab inoculation appears as:
What is red pigment is observed radiating away from the central stab line?