Lab Mental Math
To make 15 mL of a primary antibody solution at 1:10,000, one would need to add this volume of the primary antibody to the blocking buffer.
What is 1.5 uL?
The NbSL3.3Q nanobody described in the Lange et al. paper was reported to bind to branched ubiquitin chains containing these two types of linkages.
What are K48 and K63?
In western blotting using a chemiluminescent detection system, the secondary antibody is usually conjugated to this enzyme, which produces light in the presence of a substrate and hydrogen peroxide.
What is horseradish peroxidase?
This amino acid is rarely found in secondary structural elements because it is conformationally restricted due to its cyclic side chain being bonded to the main chain nitrogen atom of the polypeptide.
What is proline?
To set up a 100 uL ubiquitination reaction with 100 nM of UbcH7 using a 1 uM stock solution of UbcH7, one would need to add this volume of the UbcH7 stock solution to the reaction.
What is 10 uL?
This DUB associates with the proteasome by binding to RPN13 and has specificity for cleaving branched ubiquitin chains containing a K48 linkage.
What is UCH37?
In this mass spectrometry-based technique, a protease from the hand-foot-and-mouth disease virus is used to cleave ubiquitinated proteins in a way that leaves the ubiquitin molecules intact.
What is ubiquitin-clipping?
The two most common types of secondary structures, the alpha helix and the beta sheet, are stabilized by this type of noncovalent bond, which forms between main chain atoms of the polypeptide backbone.
What are hydrogen bonds?
In preparing 500 mL of destaining solution containing 10% methanol and 50% acetic acid, this volume of deionized water would need to be added to make the solution.
What is 200 mL?
The JAMM family DUBs, which consists of 12 different members, are metalloproteases that require this divalent metal ion for catalytic activity.
What is zinc?
This microscopy technique is used to study the diffusion of a molecule in a membrane and involves measuring the recovery of a fluorescently tagged version of the molecule within a bleached region of the membrane.
What is FRAP (fluorescence recovery after photobleaching)?
In this type of graphical plot, phi and psi, the allowable rotation angles of the bonds flanking the peptide bond in a polypeptide are represented.
What is a Ramachandran plot?
To set up a restriction digest with 20 units of enzyme, one would need to add this volume of the enzyme to a 50 uL digest if the stock concentration of the enzyme is 10,000 units/mL.
What is 2 uL?
Most DUBs are classified as a member of this family of proteases because they contain an active site thiol group that attacks the scissile isopeptide bond.
What are cysteine proteases?
In the cloning of recombinant DNA molecules, this enzyme is used to covalently join the ends of two DNA segments, resulting in the formation of a recombinant (hybrid) DNA molecule.
What is DNA ligase?
These two aromatic amino acid residues absorb light at a wavelength of 280 nm, which makes it possible to determine the concentration of a purified protein in solution based on its absorbance at this wavelength.
What is tryptophan and tyrosine?
To prepare 500 mL of a 1 M solution of NaCl (formula weight = 58.44 g/mol), one would need to add this number of grams of solid NaCl to make the solution.
What is 29.22?
This family of DUBs consists of over 50 different members and is relatively nonspecific in terms of the types of ubiquitin chains it cleaves.
What are the USP (ubiquitin-specific protease) family DUBs?
In this PCR-based technique, gene expression can be measured using reverse transcription and sequence-specific primers designed to amplify target DNA in the presence of a fluorescent probe that binds to DNA.
What is real-time PCR (or quantitative PCR)?
In an alpha helix, there are this many amino acid residues per turn of the helix, which results in every third of fourth residue of the helix being very close to each other three-dimensionally.
What is 3.6?