Ex. 5
If a cell has completed the first meiotic division and is just beginning meiosis II, which of the following is an appropriate description of its contents?
A. It has half the amount of DNA as the cell that began meiosis.
B. It has the same number of chromosomes but each of them has different alleles than another cell from the same meiosis.
C. It has half the chromosomes but twice the DNA of the originating cell.
D. It has one-fourth the DNA and one-half the chromosomes as the originating cell.
E. It is identical in content to another cell from the same meiosis
A. It has half the amount of DNA as the cell that began meiosis
What are different forms of the same gene called?
alleles
B+ blood agglutinates with the addition of what?
B antiserum and Rh antiserum
Which of the following statements is INCORRECT?
A. Proteins do not contain phosphorus.
B. In DNA, purines always pair with pyrimidines.
C. In DNA, the bond between nitrogenous bases is stronger than the bond between sugar and phosphate groups.
D. None of the above
C. In DNA, the bond between nitrogenous bases is stronger than the bond between sugar and phosphate groups
Which of the following properties of Taq’polymerase allows setting up a PCR experiment?
A. It’s produced from bacteria that live in cold conditions
B. It’s produced from bacteria that can thrive under extremely hot environments
C. It has a different function than that of mammalian polymerases
D. None of the above
B. It’s produced from bacteria that can thrive under extremely hot environments
During what stage of mitosis are chromosomes usually photographed in the preparation of a karyotype?
metaphase
Why did the majority of seedlings grown in the dark appear albino?
environmental factors affect phenotype
If a person produces B antibodies, what could their blood type be?
Type A or Type O
After Miescher’s work, which of the following could NOT be hypothesized to be the molecule that makes up genetic material?
A. Proteins
B. Phosphorous
C. Nuclein
D. Lipids
E. Phosphorous and Lipids
E. Phosphorous and Lipids
The “Alu” inserts in our DNA
A. Produce proteins that are needed for DNA replication
B. Are coding DNA that are not needed for homeostasis
C. Have a function in homozygous (+/+) individual and no function in a homozygous (-/-) individual
D. Are just non coding DNA that are not required for homeostasis
E. None of the above
D. Just non coding DNA that are not required for homeostasis
What is the area of active cell division in plants know as?
meristem
In a test cross, crossing occurs between an unknown individual and a known. What is known about the genotype in a test cross?
the known parent must be homozygous recessive
In a paternity test, the mother has type A+ blood and her son has type O- blood. If the husband has type B+ blood can he be the father?
Yes, he might have heterozygous Rh factor
What deoxyribonucleotides bind to each other?
Cytosine and Guanine
Thymine and Adenine
How does gel electrophoresis separate DNA fragments?
by size
How can we quantify the amount of time a cell spends in various stages of mitosis?
Mitotic Index (divide the number in each stage by the total number of cells counted)
How many Degrees of Freedom would there be in a Chi-square test for a dihybrid cross? (DoF = C – 1)
3 Degrees of Freedom (4 categories–1=3)
A pregnant woman is having complications and is told by her doctor that this is due to the Rh factor found on blood cells. What are the identities of the woman and her fetus?
Mother is Rh-, fetus is Rh+
According to Meselson and Stahl's experiment, after 2 CONSERVATIVE replications of DNA, what would be the predicted % of "heavy" N15/N15 molecules?
A. 10%
B. 0%
C. 25%
D. 50%
C. 25%
The function of forward and reverse primers in a PCR is
To bind to single stranded DNA and enable polymerase to add nucleotide
FINAL JEOPARDY: Say whether the statement is T or F. Correct False statements.
(T/F) Nondisjunction occurs during metaphase.
(T/F) Mendel’s first law states that the members of pairs of genes stay together during gamete formation.
(T/F) A person with type AB- blood needs a blood transfusion. A safe donor can have O+ blood.
(T/F) DNA replicates in a dispersive way.
(T/F) Taq’polymerase reads template strand in 5’-3’ direction, allowing daughter strand to form in 3’-5’ direction
(F) Nondisjunction occurs during anaphase I or II.
(F) Mendel’s first law states that the members of pairs of genes segregate during gamete formation.
(F) A person with type AB- blood needs a blood transfusion. A safe donor can have A-, B-, O- blood.
(F) DNA replicates in a semi-conservative way.
(F) It reads template strand in 3’-5’ direction , allowing daughter strand to form in 5’-3’ direction
You perform a Chi-square analysis and find that your calculated Chi-square value is extremely low. This would likely indicate that
The deviations in the data are not statistically significant and there is a greater chance that your results occurred randomly (fail to reject the null hypothesis)
You are given a blood sample from a crime scene and need to determine if a suspect with B- blood was responsible for the crime. Given this blood sample, as well as anti-A, anti-B and anti-Rh sera, which test would indicate that the crime scene sample matched that of the accused?
A: no agglutination
B: agglutination
Rh: no agglutination
Explain each of the Models in Meselson and Stahl’s experiment.
Image
What are the 6 components for PCR?
DNA template, primers, Taq DNA polymerase (enzyme), dNTP, Mg2+ (cofactor), buffer