Category 1
This happens to the particles when the particles that are the same density as the liquid around it.
What's does not move?
What RPM stands for.
What are revolutions per minute?
The most important thing when it comes to centrifugation.
What is BALANCE, BALANCE, BALANCE?
TRUE
True or False: Chemicals can be taken and mixed in the sink as long as they neutralize.
False -- never mix chemicals in the sink
A particle that is more dense than the liquid will do this.
What is move down the tube?
The two types of centrifugation.
What are differential and gradient centrifugation?
The type of centrifugation that separates based on density.
What is gradient centrifugation?
The phase in cell culture that has the most viable growing cells.
What is log phase?
The three steps in PCR.
What is denaturation, annealation, and extension?
A particle that is less dense than the liquid does this.
What is moves up the tube?
The three type of centrifuge?
What are benchtop (clinical) ~10,000 RPM; high speed ~10,000 - 30,000; ultracentrifuge ~80,000?
The type of centrifugation that separates based on particle size and sedimentation rate.
What is gradient centrifugation?
Hemocytometer counts cells and estimates the number of cells when it comes to organoids.
FALSE -- hemocytometer is used in cell dissociation
Temperature for denaturation in PCR.
What is 90°C?
What is relative centrifugal force or Xg?
True or False: RPM stands for rotations per minute.
False -- revolutions per minute is the better answer.
What the length of time for cell dissociation depends on.
What is the nature of the tissue and how finely the tissue has been minced?
What is 55-65°C?
Temperature for extension in PCR.
What is ~72°C?
The type of centrifuge that is ~10,000 - 30,000 RPMs.
What is high speed centrifuge?
The equation to determine RCF (relative centrifugal force).
Types of research that cell dissociation is used for (there are 5).
What is biomedical research, cell biology research, cell therapy research, stem cell research, and transplant research?
Involved in permanent silencing.
What is shRNA?
Type of silencing siRNA does.
What is transient silencing?