Cell Basics
Cell line used in BMES 3024
What is 4T1 cells?
Biosafety level of the BME Cellular Engineering lab
What is BSL-1?
Dye that can be used to assess cell viability (hint: it can be used during cell counting)
What is trypan blue?
Type of breast cancer that 4T1 cells mimic
What is triple negative breast cancer?
Type of farm animal that Dr. Tate has at home
What are chickens?
4 people who are credited with the discovery of DNA
Who are Watson, Crick, Franklin, and Wilkins?
PPE required for thawing cells in addition to lab coats and gloves
What are safety glasses?
Assay used to look at secretion of VEGF from 4T1 cells
What is an ELISA?
Chemotherapeutic agent that is used to treat breast cancer
What is doxorubicin?
Lab Ryosuke works in at Virginia Tech
What is Dr. VandeVord's lab (or the TNT lab)?
The flow of genetic information
What is bleach?
Assay performed in the fume hood since the dye is toxic to look at protein concentration
What is the Bradford assay?
IC50 level for chemotherapies is higher in this type of cell culture experiment (2D vs 3D)
What is 3D culture?
Place where Katie is from
What is Weehawken, NJ?
Cell cycle phase in which DNA synthesis occurs
What is the S phase during interphase?
What is Environmental Health and Safety?
Purpose of high temperature at the end of PCR in thermal cycler
What is to stop enzyme reaction?
Number of passages that 4T1 cells can be carried to
What is 60 passages?
Research expenditures of the BEAM department in 2024
What is 14 million dollars?
Cell counter that is used in the USLB lab to automatically count cells
What is the Cell Countess 3?
Committee at Virginia Tech that regulates biosafety labs
What is the Institutional Biosafety Committee?
Assay that uses Thiazolyl blue tetrazolium bromide to assess cell viability
What is the MTT assay?
Receptors that are missing in TNBC
What are estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2)?
What is 2001?