Aseptic Technique 1
Always wipe your hands and work area with _____?
-Disinfecting Solutions
How many times should you use a sterile pipette tip?
Only Once
Arsenical Material is incompatible with?
Any reducing agent
What is the purpose of the chemical hygiene plan?
-Provide safety and health procedures for all employees involved in lab operations
Should you wipe the outside of containers, flasks, plates, and dishes before putting them in the hood?
-Yes
Can you talk while performing sterile procedures?
No. Human saliva carries bacteria.
Fluorine is incompatible with?
All other chemicals
What document is included in the CHP?
-Hazard communicaction plan
What is a biohazard safety level?
-Designations applied to projects or activities conducted in labs in order of containment, based on the degree the health risk.
What BSL designation is MCA?
BSL 2
Acetone is incompatible with?
Concentrated nitric and sulfuric mixtures
How often should the CHP be updated?
Within 3 months of the lab receiving a new chemicals, established new processes/procedures, or obtain additional information
What does the term Aseptic Technique refer to?
What is BSL-4?
Exotic agents that pose a high risk of aerosol transmitted lab infections and life-threatening diseases
Calcium dioxide is incompatible wit?
Water
How long should the CHP hold onto and maintain records?
3 years
State 3 general rules of any aseptic tecniques.
-Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air
-Make transfers over a disinfected surface
-Start the operations only when all equipment and materials are within immediate reach
-Complete all operations as quickly as possible, but without any hurry
-Vessels must be open for the minimum amount of time possible
-The parts of sterile pipettes which will be put into cultures or sterile vessels must not be touched or allowed to come into contact with other non-sterile surfaces, such as clothing, the surface of the working area, or the outside of bottles/ test tubes
What is one example of an aseptic technique?
-Cleaning and disinfecting lab surfaces with disinfecting chemicals such as 70% EtOH, 70% IPA, or a bleach solution prior to use
-Limiting the duration that cultures or media are uncapped and exposed to the air
-Keeping samples covered whenever possible
-Effectively sterilizing equipment that comes into contact with samples and avoiding breathing on cultures or sterile instruments.
Chlorine dioxide is incompatible with?
-Ammonia
-Methane
-Phosphine
-Hydrogen sulfide
Name 3 types of records that should be in the CHP?
-Medical consultation and examination results
-Ventilation system & maintenance activities handled by 3rd party contracting agency
-Training Records
-Workplace injury records
-Hazardous waste and other environmental records
-Review and revision records