A
What is meant by activation energy?
The amount of energy which is used to initiate the reaction.
What is the active site of an enzyme?
The site where substrate binds
What factors affect enzyme activity?
Temperatur, pH, substrate
What products are obtained after lactose digestion?
Glucose and galactose
State two advantages of enzyme immobilization.
Reusable, cost efective
Describe the induced-fit model of enzyme action.
When enzyme joins with substrate, enzyme changes it's structure, it's literally adjusted to substrate. Gloves and hand.
Describe any three methods of enzyme immobilization.
Entrapment, encapsulation, cross-linking, covalent binding
What are proteases, amylases, lipases, and cellulases used for in detergents?
Remove stains of grease(lipid), stains of vegetables or grass on clothes, any stain after meal contatining protein(meat, egg, fish)
How do we immobilize beta galactosidase (lactase)?
Alginate gel, calcium bicarbonate.
The tase of lactose free milk is...... than lactose containing milk
sweeter
Name of enzyme which is used in ELISA method?
HRP
In pregnancy test what makes stripes to stain?
antibodies combines with hormone HCG
You were prescribed to take ELISA test to check for certain disease? You are lab worker and want to do the test yourself, what should you do first?
Put antibodies appropriate (complementary to )to the well, dish
add sample which was taken from human
add antibody linked to enzyme
sandwich method