Mitosis
How many daughter cells are produced, and how do they compare to the parent cell?
2, identical
How many daughter cells are created, and how do they compare to the parent cells?
4, genetically unique
What process are we replicating to make our glowing e.coli?
How insulin is made
What is your group's GMO presentation topic?
varies
How many make-up assignments can you do?
How many copies of each chromosome are there during Interphase, RIGHT before a cell enters mitosis?
2
When does crossing-over occur?
Prophase 1
In what conditions would e.coli with the pGLO plasmid GLOW
On a plate with nutrients (LB) and arabinose
Describe one use for GMOs (be specific)
Insulin, glofish (entertainment), extra food nutrients, etc.
What is a positive control?
Always see the expected result
What phase of mitosis would you see the cleavage furrow or cell plate forming?
Telophase
How many copies of each chromosome are in the daughter cells at the end of meiosis?
1
What are ALL the components of the pGLO plasmid?
ampR gene, ori, GFP gene, araC gene
What is a transgenic GMO?
Adding DNA from a different organism
What is an allele?
A different variation of a gene and influences the phenotype
How does mitosis connect to cancer?
Increased mitosis --> increased chance for mutations --> increased chance for cancerous mutations --> more cancer
What is the difference between metaphase 1 and metaphase 2
The homologous chromosomes are paired and lined in the middle of the metaphase plate. In metaphase 2, the sister chromatids (single set) are in the middle, and there are two cells
What two types of selection does are pGLO plasmid allow for?
antibiotic selection, and inducible gene expression
What is a cisgenic GMO?
Changing the gene expression of a gene already found in an organism
How would you set up one side of the punnet square for a BbPp individual?
FOIL --> BP, Bp, bP, bp