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Staining Bacteria Lab
100

What are the 3 domains?

Bacteria, Archaea, Eukarya

100

What was the first vaccine and what separate disease was it derived from?

Smallpox, Cowpox

100

What was the original goal of the Microbial Genome Project?

To sequence the whole genomes of microbes with potential applications in environmental biology, research, industry, and health

100

What are the two most commonly used methods to identify microbial strains?

PCR and Whole genome sequencing

100

What color does Gram-positive bacteria appear?

Dark purple

200

What is the bacterial cell wall made of?

Peptidoglycan
200

Give 3 biological tools used during the adaptive immune response.

B cells, T cells, Plasma cells, Antibodies, Macrophages
200

How did scientists extract DNA from all of those microbes?

Usually scientists isolated DNA directly from the environmental sample because it was too difficult to replicate the growth conditions of all of the different microbes studied.

200

What is the branch of public health sciences that studies the incidence, distribution, and control of diseases and other factors relating to health

Epidemiology

200

What is the decolorizer?

Alcohol

300

What is corkscrew bacteria called?

Spiral
300

How do vaccines work?

Vaccines insert part of a virus into an organism to stimulate an immune response, allowing the organism to generate memory B cells for this specific antigen

300
Give 3 potential goals of the Human Microbiome Project.
  • To determine if individuals share a core human microbiome

  • To understand how we acquire and maintain microbial communities

  • To identify how changes in the microbiome correlate to changes in human health

  • To identify the conditions that affect the microbiome

  • To develop new methods to support analysis of microbiomes

  • To discuss the ethical, legal, and social implications of studying this branch of science

300

Bioinformatics tool for identifying microbes made in 1996

PulseNet
300

What is the counterstain?

Safronin O
400

What is a fusion protein?

Recombinant proteins tagged with a known protein, acting as a probe.

400

What is an inactivated vaccine?

Dead or inactive microbes are inserted into the organism to stimulate an immune response

400

What did we learn from the Human Microbiome Project?



  • There are about 1000 bacteria strains present in each person

  • The microbiome starts at birth and is partially acquired from the biological mother

  • The microbiome is substantially different from person to person

  • The gut microbiome remains relatively stable over time in individuals

400

What are gene chips used for?

to detect and identify pathogens and examine host responses to infectious diseases

400

Which type of bacteria has a complex cell wall and low peptidoglycan?

Gram Negative

500
What does Luciferase act as and produce?

A reporter gene, bioluminescence

500

What is a disadvantage of an attenuated vaccine?

Sometimes the weakened virus can mutate and grow in strength.

500

How many bacteria strains are present in each person approximately?

1000 

500

What type of transmission involves a pathogen being passed from mother to child in utero or during childbirth?

Vertical Transmission

500

What acts as the reagent?

Gram Iodine

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