Mass Analyzers
Four parallel rods
What is a quadropole?
Soft, liquid, Taylor Cone
What is ESI?
One may argue this is the most common detector
What is an electron multiplier?
These large molecules do many things - catalyze reactions and are straight-forward to sequence by MS/MS
What are proteins?
Used for crystallized or dried samples.
What is MALDI-MS? (Matrix Assisted Laser Desorption Ionization Mass Spec)
If you were an ion you may ask "Are we there yet?"
What is Time-of-flight?
Mass spectra generated from this method are usually compared to the NIST database
What is EI? (Electron impact/electron bombardment)
This detector converts electrons to light after they hit a phosphorous screen
What is a photomultiplier?
Sweet and best analyzed by GCMS after derivatization.
What are sugars?
This mass-spec technique first separates compounds based on their boiling point
What is GC-MS? (Gas-chromatography mass spec)
Ions flying around a small magnet, made by Thermo Fisher
What is an Orbitrap?
This hard-ionization source is hot, 6000 K hot!
What is ICP? (inductively couple plasma)
This mass analyzer had a collector not a detector.
What is a Calutron?
MRLHEAR or CGACLIEGK for example
What are tryptic peptides?
Used for studying the shape and stoichiometry of protein complexes
What is NMS? (Native Mass Spectrometry)
A super-conducting magnet and detector built in one.
What is a cyclotron? (FT-ICR)
If you are a solid in trouble, you could ask your friend Neo
What is MALDI? (MATRIX assisted laser desorption ionization)
In a detector, these are made of Al2O3, BeO, GaP, or CsSb
What are dynodes?
These molecules generate MS/MS patterns with a characteristic separation of 14 m/z
What are lipids/hydrocabons?
I feel heavy now. I shouldn't have bathed in D2O.
What is HDX? (Hydrogen-deuterium exchange)
Paul might say "Help! I'm stuck!"
What is an ion trap?
Help! I'm a liquid under attack by Xenon!
What is FAB? (Fast Atom Bombardment)
Back in the day, rock stars like JJ Thomson used these instead of electronic detectors.
What is a photographic plate (film)?
This class of biomolecules comes from Greek for "change"
What are metabolites?
Using 3 quadrupoles, this targeted method only monitors a single precursor to product ion transition.
What is SRM? (Selected Reaction Monitoring)