Mol Biol
Mol Biol
Mol Biol
Mol Biol
Mol Biol
100
It causes a change of a nucleotide to a different one.
What is a point mutation?
100
The building block of nucleic acids
What is a nucleotide?
100
Small circular dsDNA found within many bacteria.
What is a plasmid?
100
This is the nucleotide that always pairs with a pyrimidine.
What is a purine?
100
Watson and Crick (together with Franklin and Wilkins)
Who discovered the structure of the dsDNA?
200
It causes a premature STOP codon
What is a nonsense mutation?
200
This mutation does not change the phenotype of the individual
What is a silent mutation?
200
It causes a frameshift mutation
What is an insertion/deletion?
200
This is the bond that always holds the dsDNA helix together.
What is hydrogen bonds?
200
ATG
What is the start codon?
300
UAA, UAG, UGA
What are stop codons?
300
It will allow to make many copies of a sequence of DNA and to express a large amount of a protein.
What is cloning?
300
It serves as a template for the ribosome
What is mRNA?
300
It is a technique used to separate DNA fragments of different sizes.
What is electrophoresis?
300
This technique can make many copies of a fragment of DNA in a short amount of time.
What is PCR?
400
AAUAAA
What is the signal for signal and cleavage?
400
Template, 2 DNA primers, dNTPs, Heat-stable DNA-pol
What components are required for a PCR?
400
ssDNA without introns produced from mRNA via retrotranscription.
What is cDNA (complementary DNA)?
400
It is cut out from RNA before it can be translated in eukaryotes.
What is an intron?
400
EcoRI is purified from this organism.
What is Escherichia coli?
500
This is the number of A nucleotides in a 100 bp dsDNA with 40 C nucleotides.
What is 10?
500
This is the number of G nucleotides in a 100 bp dsDNA with 15 T nucleotides.
What is 35?
500
CATATG
What is an example of a palindromic sequence?
500
DNA runs in this direction in an electrophoretic gel.
What is positive pole?
500
These are the three steps of PCR (in order).
What is denaturation, annealing and extension?
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