Poisoning
Bacteria
Mycobacterium
Biochemical Tests
Spore staining & some tests
100
Anaerobic sporeformers
Clostridia species
100
Can pass via milk from infected cow
Wildlife such as deer are infected
M. Bovis
100
Red = +
Yellow = -
(Positive means production of mixed acids by bacteria)
Methyl Red
100
(dye carbol fuchsin (red) used during heat step)
Red to red-black = Spores
Light gray to white = Cells
Dorner
200
Food poisoning found in chicken and eggs
Salmonella
200
All are clubed shaped rods
Arranged in palisades, Vs or chinese letters
Diphtheroids residents
The pathogen causes diphtheria
Prevented by what vaccine?
Corynebacterium diphtheriae
Prevented by the vaccine DTAP
200
Both can cause Tuberculosis
Mycobacteria tuberculosis & M. bovis
200
Blue = +
Green = -
(Change of green to blue means microbe was able to utilize it as its sole source of carbon)
Simmons Citrate
200
Lactose + = Pink (with diffusible or non-diffusible pigment)
Lactose - = Tan color
MacConkey's Plate
300
Usually a protective resident
Can cause tooth decay
In vaginal area, it poduces a low level of acids to protect against yeast infection
In intestine, it produces reuterin, a chemical that inhibits growth of E. coli & Listeria
Lactobacillus
300
Skin tests for Tuberculosis
Vaccine for TB?
Skin Tests
PPD
OT
Mantoux (Bubble test)
Vaccine
BCG
300
Pink = +
Yellow = -
(Positive means breakdown of urea by urease enzyme, to produce ammonia)
Urease Test
300
(dye malachite green used during heat step)
Green = Spores
Red = Cells
Schaefer Fulton
400
Food poisoning found in rice
Bacillus Cereus
400
Gram negative
Dark metallic green/glack colonies
Non-spore forming
Ferment lactose
In animal & human intestines
Presence of it means sample has feces in it
100 ml of sample
An example of it is E. coli
Coliforms
400
Have mycolic acid (a lipid) in Cell wall
Are slow growing bacteria
(red = ____bacteria/ blue = any other bacteria in the world on Acid Fast Stain)
Mycobacteria Species
400
Red = -
Yellow = +
(Positive means fermentation of sole carbon source glucose & production of acids)
Phenyl Red Glucose
400
Red ring = +
Any other color = negative
Detects production of butanediol
VP (Vogues Proskauer)
500
Food poisoning, deli meat, & Ice cream
Is coccobacillus (some think looks like cocci/ some like rods)
Can grow in refrigerator & freezer
Listeria monocytogenes
500
3 Representatice Bacteria
Corynebacterium
Listeria
Lactobacillus
500
Has egg whites - Enrich media & help Mycobacteria grow better
Has malachite green - To inhibit growth of other bacteria
Special medias to selctively grow Mycobacteria
500
Red ring = +, indole
Black core = +, sulfide
Yellow ring = -, no indole
SIM (Sulfide Indole Motility)
500
EMB plat = Lactose - (colonies appear clear & like water droplets)
MacConkey Plate = Lactose -
Citrate = Green -
Irease = + (hot pink)
H2S = + (development of black with SIM)
Proteus