Stains
Dyes used for capsule stain.
What is Congo red and maneval's solution?
Components of KF-strep agar
What is sodium azide and TTC?
Expected results for a fecal coliform inoculated in LST-MUG broth.
What is growth, gas and fluorescence?
Released from the sporangium.
What is spores?
Acquired resistance.
what is resistance that develops through mutations?
The pink cells seen from an endospore stain.
What is vegetative cells?
Medium and reagents used for ammonification test.
What is 4% peptone broth (medium) and OPA/TCA (reagents)?
Frosted glass/granular colony on mitis-salivarius agar.
What is S. mutans?
Arthrospores
what is made from fragmented hyphae and will develop into the mold?
Controls for endospore stain.
What is bacillus subtilis.
The stain that doesn't use heat (heat fix, slide warmer).
What is capsule stain?
The difference between LST-MUG broth and EMB agar?
What is presumptive test for E. coli (LST-MUG) and confirmation test for E. coli (EMB agar)?
Features of coliforms.
What is bacillus, gram negative, facultative anaerobe, non-spore forming, lactose fermentation (48 hours at 35 degrees Celsius)?
Type of reproduction for yeast
What is asexual reproduction?
Reagent added to starch agar post incubation and expected color change.
What is gram's iodine and dark purple color if amylose is present?
Only purple cells was viewed on gram stain.
What is decolorizer (ethanol) wasn't left on long enough?
Components of mannitol-salts agar
What is 7.5% NaCl, mannitol, and phenol red?
Bacteria that result in red versus yellow agar color in mannitol-salts agar (skin swab)
What is S. epidermidis (red) and S. aureus (yellow)?
The four molds tested via henrici slide.
What is geotrichum, aspergillus, penicillium, mucour?
The four modes of action for antibiotics.
What is inhibition of cell wall synthesis, inhibition of translation, inhibition of nucleic acid synthesis, cell membrane disruption?
The results for Gram stain of the controls when Gram's iodine was forgotten.
What is pink bacilli and pink cocci?
Components and purpose of each for Mitis-salivarius agar.
What is tellurite (selective against gram negative, most gram positive, and many molds. Differential by reducing Enterococci into black colonies), crystal violet (selective: gram positive), trypan blue dye (differential: absorbed by streptococcus to form blue colonies), and sucrose (differential: metabolized and produces large and mucoid colonies)?
Reason for colonies being red on KF-strep agar.
What is reduction of TTC to formazan from cell's anaerobic metabolism?
Protocol for Henrici slides
What is add ripped up paper towel into sterile plate, get wet with deionized water, break wooden dowel and place into plate in V shape, sterilize slide (via ethanol and flaming), add a couple drops of molten SAB, inoculate with spores, place cover slip, and incubate?
What is nitrate reduction to mystery product?