Media
Name a media that is selective AND differential for gram negative bacilli
EMB
MAC
XLD
What is an OPTIMAL wound specimen for a culture of suspected anaerobic organisms?
A syringe of aspirated pus collected BEFORE antibiotic administration.
What organism appears beta hemolytic on SBA and is GPC in clusters?
S. Aureus
How long are routine Lab cultures held in the lab?
24-48 hrs
Sputum sample:
10 WBC & 50 EPI/lpf
NOT acceptable
Which media would you inoculate for a stool specimen? (at least 3)
SBA
MAC
Campy
HEK
XLD
What is the most convenient type of collection for a urinalysis?
Clean catch
How does lactose fermentation with E.Coli appear on HEK?
Orange
What temp is yersinia agar incubated at?
22c
Urine sample collected at 0800 on L&D and delivered to the lab at 1500?
NOT acceptable
Which media is a general-purpose media?
SBA
DAILY DOUBLE:
Calculate the colony count to be reported:
1uL loop used
25 colonies present on SBA
On HEK how is H2S shown on colonies?
appear black
What temp is campylobacter agar incubated at?
42
Sputum:
25 WBC & 5 Epi/lpf
Acceptable
Which medias would you inoculate for a wound specimen?
SBA
MAC
Choc
LJ
How does Salmonella appear on XLD?
Red with black centers
If bacteremia is suspected which type of culture should be collected?
Blood
CBC and Troponin ordered STAT at 0800, collected at 0805, and delivered to the lab at 0815
Acceptable
Chocolate agar is used to isolate haemophilus because it has?
X & V factor
When isolating Group D strep which media should be used?
BEA
How is lactose fermentation shown on MAC?
Hot pink colonies
DAILY DOUBLE
Which organisms are most susceptible to a delay in the laboratory?
A urine sample delivered to the lab in a CBC tube
Unacceptable