Metabolomics/Lipidomics
Study of all small molecules (metabolites or lipids) in biological systems.
What is metabolomics/lipidomics?
Mass spectrometry data with low mass accuracy measurements (single unit mass measurement).
What is low resolution mass spectrometry?
Academic research groups studying small molecules in biological systems.
Who commonly carries out metabolomics or lipidomics research?
Customer currently uses Q-TOFs for the untargeted analysis of metabolites and lipids.
How do you deal with in-source fragmentation?
How good is your mass accuracy?
How good is your data quality?
Exploris series (Exploris 120, 240) with Acquire-X, and Compound Discoverer.
What solutions (hardware and software) can we position against Q-TOFs (if the customer's main priority is NOT throughput)?
Workflow used to analyse unknown metabolites or lipids within a sample.
Untargeted metabolomics or lipidomics/metabolic or lipid profiling.
Mass spectrometry data with high mass accuracy measurements (several decimal point mass measurement).
What is high resolution mass spectrometry?
Academics offering a service to analyse small molecules in biological systems.
Who commonly offers a service to carry out metabolomics or lipidomics research?
Customer's main priority is throughput.
What is your current workflow?
Do you require multiple fragmentation strategies?
Do you require fragmentation of each sample individually?
Orbitrap Astral with Compound Discoverer.
What solutions (hardware and software) can we position if the customer's main priority IS throughput?
Workflow used to quantify a subset of known metabolites or lipids.
Targeted metabolomics/lipidomics.
Type of mass spectrometry used for the analysis of known metabolites and lipids (targeted metabolomics/lipidomics).
For which analyses is low resolution mass spectrometry sufficient in metabolomics and lipidomics?
Non-academics such as CROs can offer metabolomics and lipidomics services.
Which non-academic groups commonly provide metabolomics or lipidomics services?
Customer is interested in structural elucidation.
Do you have a current structural elucidation workflow?
Is spectral library matching sufficient?
Orbitrap IQ-X with Compound Discoverer and Mass Frontier.
What solutions (hardware and software) can we position against if the customer's main priority is structural elucidation.
Workflow to investigate the biotransformation of a single or handful of metabolites.
Metabolite identification (MetID)
Type of mass spectrometry used for the analysis of unknown metabolites and lipids (untargeted metabolomics/lipidomics).
For which analyses is high resolution mass spectrometry required in metabolomics and lipidomics?
Groups/companies doing "multi-omics" analyses or targeted metabolite or lipid analysis.
What are some clues that a company or research group may be interested in expanding to metabolomics or lipidomics?
Customer is interested in throughput and sensitivity.
Orbitrap Astral with Compound Discoverer
Workflow to BOTH analyse unknown metabolites/lipids and quantify a subset of known metabolites/lipids.
Simultaneous quantitation and discovery (SQUAD)/semi-targeted analysis.
Type of mass spectrometry used for the analysis of BOTH known and unknown metabolites and lipids (targeted and untargeted metabolomics/lipidomics).
Which type of mass spectrometry allows the measurement of both known and unknown metabolites/lipids?
Customer is interested in targeted and untargeted analysis.
Do you require simultaneous targeted and untargeted analysis?
How do you currently perform your targeted analysis?
Exploris series (Exploris 120, 240) with Acquire-X, Compound Discoverer, and TraceFinder.