QC Metrics
What metric shows total number of reads uploaded?
What is total reads?
What step trims adapters and removes low-quality reads?
What does high rPM indicate?
What is high abundance of organism?
What platform is used for mNGS analysis in this workshop?
What is CZ ID?
What would high host reads suggest about a sample?
What is high host contamination?
What metric reflects good sequencing quality after filtering?
What step removes host sequences?
What is HISAT2/Bowtie2?
What value compares microbes to background?
What is Z-score?
What tool helps visualize taxa across samples?
What are heatmaps?
What would low QC reads suggest?
What is poor sequencing quality?
What does ERCC read count help evaluate?
What is input RNA amount?
What issue causes many short reads in fastp?
What are adapter primers or degradation?
What does long alignment length suggest?
What is strong or reliable match?
What tool is used for sequence alignment outside CZ ID?
What is BLAST?
What might cause many adapter dimers?
What is poor library prep?
What does subsampled fraction indicate?
What is the ratio of reads used after filtering?
What happens if reads are low complexity?
What is repetitive sequences or poor quality?
What does percent identity measure in reference to your sequence and the database of choice?
What is sequence similarity?
What platform helps review sequencing run quality?
What is basespace?
If all reads are “undetermined,” what is likely wrong?
What is index or sample sheet issue?
What happens if Passed Filters is very low?
What are Many reads lost during QC?
What does duplicate removal improve?
What is data quality and uniqueness?
Which CZ ID metric ranks microbial hits based on abundance, Z-score, and alignment evidence?
What is combined NT/NR score or CZ ID score?
What platform helps review sequencing run quality?
What is SAV (Sequencing Analysis Viewer)?
What is the ultimate goal of mNGS data analysis?
What is identify and interpret potential pathogens in a sample?