Plating Methods
Used to count colony growth on media plates
What is Standard Plate Count?
Focuses on two molecules
What is Antigen & Antibody?
Genetic characteristics of an organism
What is Genotypic methods?
Two single stranded DNA from two different sources bind together
What is Hybridization?
- GENE track assay
- API
- Petrifilms
What are common Rapid methods?
A mechanical device that distributes the liquid sample on the surface of a rotating plate.
What is Spiral Platter
Direct, Indirect and Sandwhich
What is Types of ELISA?
Expressed characteristics of an organism
What is Phenotypic methods?
DNA, rRNA, mRNA
Classification of bacteria based on biochemical analytics.
What is Anayltical Profile Index (API)?
Used when the researcher knows that the sample has very few microorganisms
What is Drop method?
If bacteria is present in the sample then the antibody and antigen will bind causing a color change.
What is Direct ELISA?
- PCR
- Ribotyping
- Pulsified-field Gel electrophoresis
-Nucleic Acid Probe
What are the most common Genotypic methods?
ssDNA from two different sources is allowed to reanneal to form dsDNA
What is hybridization?
Used to detect foodborne pathogens but is not used as much in the food industry because it gives a lot of false positives/negatives.
What is gene track assay?
Can be used to detect viable and non-viable cells
What is Direct Microscope count?
Enzyme is not bound with primary antibody but with the secondary enzyme.
What is Indirect ELISA?
-Morphology
-Staining
- Resistance to antimicrobials
- Fatty acid methyl ester (FAME) profiles
- Serotyping
-Bacteriophage typing
What are the common Phenotypic methods?
To quantify microorganisms
What is Real Time PCR?
The identity and order of nucleotides in the genetic materials
What is genome sequencing?
Used to detect viable cells but not a precise method of analysis
What is MPN (most probable number)?
- Antigen is captured by antibody coated support
What is Sandwich ELISA?
_____ characteristics of an organism is more stable, than _____ characteristics.
What is genotypic(1), and phenotypic(2).
a short single stranded sequence, complimentary to known sequence
What is Probe?