In the Media
This is the substrate in the milk plates
Casein
This enzyme breaks down Gelatin
gelatins
This is the term for an organism that can grow above 70°C
Extreme (obligate) Thermophile
This color indicates a positive Citrate Slant test
Blue
This is the formula for calculating CFUs
Colonies/plate x Dilution factor x 1/mL plated = CFU/mL
This media is used to test the anaerobic capabilities of bacteria
Thioglycollate
This enzyme breaks down Tryptophan
Tryptophanase
This is a term for an organism that can grow in Arctic Ice
Psychrophile
This color indicates a positive Hydrogen Sulfide test
When I am grown at room temperature, I grow beautiful red colonies
Serratia marcescens
This media contains a substrate for lipase to act upon
Tributyrin Agar
This enzyme breaks down hydrogen peroxide
Catalase
This organism is a big fan of salt
Halophile
After inoculation, this broth is split to test 2 tests
MR-VP broth
I am the thermophile we used in class
Bacillus stearothermophilus
This is the fat source in LBS (lactobacillus) agar
Polysorbate 80
This enzyme brings citrate into the cell
Citrate Permease
This is the term for an organism who likes to live in pH below 6
These are the two chemical names for Barritt’s Reagents A & B.
KOH & α-naphthol
This extreme group of bacteria is the reason we have PCR today
Thermophiles
These are the three carbohydrate sources in the fermentation broths
glucose/dextrose, sucrose, lactose
This enzyme converts lysine into cadaverine and carbon dioxide
Lysine Decarboxylase
Most of the organisms we use in class fit into this temperature category
Mesophiles
This enzyme converts phenylalanine to phenylpyruvic acid and ammonia
Phenylalanine deaminase
This is the reason why mineral oil is needed during Decarboxylase reactions
Reaction must occur anaerobically