Name the Test
Name the Bacteria
Chemical Reactions
Interpret the Test
In the Lab
100
Tells you if the bacteria has proteins for movement.
What is a motility test?
100
Your EMB plate shows a metallic green color.
What is E. coli?
100
Citrate test- ask what it tests for.
What is import and utilize citrate?
100
The most likely ID of the unknown in this photo:
What is E. coli?
100
Your instructor for intro to micro lab and lecture.
Who is Dr. Jordan?
200
Agar contains dyes eosin Y and methylene blue.
What is Eosin Methylene Blue (EMB) test?
200
Your HE plate shows a black precipitate.
What is S. typhimurium?
200
SIM test result: red kovac's reagent and yellow media. Ask what chemical reaction took place.
What is breakdown of tryptophan into indole?
200
Bacteria that grow on HE agar (name the genuses, not the gram status)
What is Shigella, Salmonella, and E. coli species?
200
When you put the base components into a reaction but do not put the reactive ingredient in.
What is a negative control?
300
Bile salts are the selective ingredient.
What is Hektoen Enteric (HE) test?
300
Your EMB plate shows light lavender colonies.
What is S. flexneri or S. typhimurium?
300
The product that makes urease tubes turn pink.
What is ammonia?
300
OF Unitube Result: the top chamber remains green while the bottom chamber turns yellow.
What is fermentation or anaerobic metabolism?
300
Type of media that allows only certain species of bacteria to grow.
What is selective media?
400
Tells you if bacteria use an oxidative (aerobic) metabolic pathway or fermentation (anaerobic).
What is oxidation-fermentation (OF) test?
400
Agar turns black on a bile esculin plate.
What is E. faecalis?
400
The color of precipitate formed when ferric ammonium citrate reacts with hydrogen sulfide.
What is black?
400
What is the test results for the SIM test? Test results indicated by the arrow below.
What is sulfur positive and indole positive (S+/I+)?
400
The name of the inner most type of tube:
What is a Durham tube?
500
Tells you whether the bacteria have sulfur reductase and/or tryptophanase.
What is Sulfur, Indole, Motility (SIM) Test?
500
The possible ID of the right tube if the left tube is the negative control.
What is S. typhimurium?
500
PR test result: yellow media with bubble in durham tube. Ask what chemical reaction took place.
What is fermentation with gas production?
500
On an EMB plate, bacteria turn dark purple (not light purple or metallic green).
What is slight fermentation or weak acid production?
500
The results of this test are not clear, so we have to add ______ to the tubes.
What is TTC?