Culture Media
Gram Stains
Clinical Cases
GPC
Enterobacteriaceae
100

This selective medium is used to isolate pathogenic Neisseria species.

What is Modified Thayer Martin agar?

100

Gram-positive cocci in clusters

What is Staphylococcus?

100

A blood culture grows catalase-positive, coagulase-negative cocci that are novobiocin susceptible from a patient with a prosthetic heart valve.

What is Staphylococcus epidermidis?

100

This species is novobiocin resistant.

What is Staphylococcus saprophyticus?

100

All Enterobacteriaceae ferment which sugar?

What is glucose?

200

This enriched medium supplies X and V factors.

What is Chocolate agar?

200

Gram-positive cocci in chains

What is Streptococcus?

200

A college freshman presents with meningitis. CSF reveals oxidase-positive Gram-negative diplococci that ferment glucose and maltose.

What is Neisseria meningitidis?

200

This alpha-hemolytic organism is optochin susceptible.

What is Streptococcus pneumoniae?

200

KIA results: A/A, gas +, no H₂S. Which organism?

What is E. Coli?

300

This medium (abbreviation only) produces a metallic green sheen for one lactose fermenter.

What is EMB?

300

Kidney bean-shaped intracellular Gram-negative diplococci

What is Neisseria gonorrhoeae?

300

Which Staphylococcus species is most associated with toxic shock syndrome?

What is Staphylococcus aureus?

300

This enzyme identifies Staphylococcus aureus.

What is coagulase?

300

This species swarms.

What is Proteus?

400

This selective medium (abbreviation only) grows Legionella pneumophila.

What is BCYE?

400

Sputum Gram Stain with > 20 epithelial cells, acceptable: Yes or No?

What is no?

400

A patient has kidney stones. The isolate swarms and is urease and H2S positive but indole negative.

What is Proteus mirabilis?

400

This species grows in 6.5% NaCl and hydrolyzes bile esculin.

What is Enterococcus?

400

This organism produces H₂S and black colonies on HE agar.

What is Salmonella?

500

Name TWO organisms commonly isolated on Hektoen Enteric (HE) agar.

What are Salmonella and Shigella?

500

Curved Gram-negative rods from stool culture

What is Campylobacter jejuni?

500

Neonate blood culture shows a beta-hemolytic, CAMP positive, catalase negative organism.

 What is Group B strep?

500

This sugar differentiates a GPC that hydrolyzes bile esculin and grows in 6/5% NaCl.

Arabinose

500

This lactose fermenter is nonmotile and appears mucoid on a MAC agar.

What is Klebsiella pneumoniae?

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