Microscopy & Stains
If the ocular = 10X and the objective = 40X, what is the total magnification?
What is 400X?
He disproved spontaneous generation with his swan-neck flask experiment.
Who is Louis Pasteur?
What are the three domains of life?
What are Bacteria, Archaea, and Eukarya?
Gram-positive bacteria have (thick/thin) peptidoglycan and (do/do not) have an outer membrane.
What is thick peptidoglycan and do not have an outer membrane?
What does the “ABC” in ABC transporter stand for?
What is ATP-Binding Cassette?
What is the total magnification under oil immersion (100X objective)?
What is 1000X?
He established postulates linking microbes to disease.
Who is Robert Koch?
The basic features found in ALL cells include cytoplasm, a cytoplasmic membrane, DNA, and ___.
What are ribosomes?
The outermost layer of the cell envelope, if present, is the ___.
What is the capsule or slime layer (glycocalyx)?
Name one of the three components of an ABC transporter.
What is substrate-binding protein, transmembrane transporter, or ATP-hydrolyzing protein?
Name one example of a differential stain.
What is Gram stain or Acid-fast stain?
He coined the term “virus” as a filterable infectious agent
Who is Martinus Beijerinck?
The microorganism thought to have oxygenated Earth’s early atmosphere.
What are cyanobacteria?
Archaeal membranes are unique because they have ___ linkages instead of ester linkages.
What are ether linkages?
What type of transport uses energy from the proton motive force instead of ATP?
What is simple transport (symport/antiport)?
This microscopy technique uses a special condenser to visualize unstained, living cells.
What is phase-contrast microscopy?
He developed enrichment culture techniques.
Who is Martinus Beijerinck?
This molecule is commonly used to build microbial phylogenetic trees.
What is 16S rRNA?
In Gram-positive bacteria, this polymer gives structural support and binds cations.
What are teichoic acids?
The “litho-” in chemolithotrophy means the use of what as electron donors?
What are inorganic molecules?
Which stain colors acid-fast bacteria red/pink?
What is carbol fuchsin?
He discovered chemolithotrophy and the role of soil microbes in nutrient cycling.
Who is Sergei Winogradsky?
Which two domains of life are most closely related?
What are Archaea and Eukarya?
This molecule is unique to the outer membrane of Gram-negative bacteria.
What is lipopolysaccharide (LPS)?
Name one inorganic molecule a chemolithotroph might use as an energy source.
What is H₂, NH₃, Fe²⁺, or H₂S?