Hepatitis A and E Diagnosis
What is the primary method for detecting Hepatitis A virus in stool samples?
ELISA
What diagnostic test is commonly used for rapid detection of Rotavirus in stool?
Rapid antigen detection tests
What is the common name for Norwalk Virus?
Norovirus
What type of genome does Astrovirus have?
Positive-sense single-stranded RNA
What is the primary use of RT-PCR in virology?
Detecting viral RNA.
Which serological marker is used to confirm recent Hepatitis A infection?
Anti-HAV IgM.
Which age group is most affected by Rotavirus infections?
Children under 5 years old.
What is the incubation period for Norovirus infections?
12-48hours
What sample is primarily used to diagnose Astrovirus infection?
Stool sample
What staining technique is used to visualize viruses in cell cultures?
Immunofluorescence staining.
Which molecular test is most sensitive for detecting Hepatitis E virus RNA?
RT-PCR
Which molecular test can confirm Rotavirus genotypes?
RT-PCR
Which cell culture method is used for Norovirus diagnosis?
Cannot be cultured
Which microscopy method can visualize Astrovirus particles?
Electron microscope
Task :-if you don't do your point goes to opponent
Select anyone
1 2 3
1. Dance
2.25 pushups
3. Song
Which sample types are commonly used for diagnosing Hepatitis E?
Serum and stool samples
Name the structural protein targeted by most Rotavirus diagnostic assays
VP6 protein.
Which genetic groups of Norovirus are most associated with human disease?
Genogroup I (GI) and Genogroup II (GII).
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What does a positive IgM test indicate in viral diagnosis?
Acute or recent infection.
What is the major route of transmission for both Hepatitis A and Hepatitis E?
Fecal-oral route.
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Symptoms of norwalk virus :
Symptoms:Nausea, vomiting, watery diarrhea, abdominal cramps.
Mild fever, fatigue, and muscle aches in some individuals.
Name the most common symptoms of Astrovirus infections.
Diarrhea, nausea, and vomiting.
What is the role of next-generation sequencing (NGS) in virology?
Identifying and characterizing viral genomes.