Microbiology

Who am I

100

Gram negative dipplococci

Kidney or coffee bean shaped

Organisms seen intracellular on gram stain

Can be normal respiratory flora

Utilizes glucose and maltose

Oxidase positive

Neisseria meningitidis

200

I cause whooping cough

I have three stages: Catarrhal stage, Paroxysmal stage, and Recovery stage

Gram negative pleomorphic coccobacilli

Grow best on Bordet-Gengou medium

Bordetella pertussis

300

Gram negative bacillus

Coccobacilli – pleomorphic

X and V factors required

Positive Quellung test

Haemophilus influenzae type B

400

CausesPontiac fever and atypical pneumonia

Gram-negative pleomorphic coccobacilli

Growth on charcoal yeast agar with iron and cysteine

Legionella pneumophila

500

Oxidase and catalase positive

Pleomorphic coccobacilli

Bipolar staining

Normal inhabitants of animal mouths

Endotoxin, cytotoxin, surface adhesins and a capsule

Pasteurella multocida

Who am I

100

Cause undulating fever

Pleomorphic coccobacilli

Caused from ingestion of imported goat cheese that is poorly pasteurized or unpasteurized

Brucella species

200

Gram negative diplococci

Oxidizes glucose only

Causes genital infections

Neisseria gonorrhoeae

300

Gram negative bacillus

Curved

Growth on Campy plate

Growth at 42 C

Urease negative

Campylobacter jejuni

400

Gram negative pleomorphic coccobacilli

Needs cysteine for growth

Carried in rabbits

Francisella tularensis

500

Gram negative diplococci

Oxidase positive

Moves like hockey puck acrossed agar

Does not ferment or oxidize glucose, maltose or sucrose

Moraxella catarrhalis

Quiz 1

100

What species are most associated with infections in humans?

A. H. influenzae

B. H. aegypticus

C. H. ducreyi

D. All the above

D

200

Which organism is non hemolytic on SBA but hemolytic on human blood?

Gardnerella vaginalis

300

A mother brings in her lethargic, feverish baby. The baby is diagnosed with meningitis and the doctor orders a spinal tap. CSF showed many tiny gram-negative rods. What is the probable cause of infection?

A. H. influenza type B

B. H. parainfluenzae

C. H. aegypticus

A

400

A medium that will sustain the growth of most Haemophilus species is?

Chocolate agar

500

The lab receives a blood culture from a vegan that is very ill. The blood culture results: small, gram negative coccobacilli and growth after 48 hours on SBA with small, smooth raised colonies. What should the microbiologist do next?

A. Consider the growth contamination

B. Perform biochemical tests to test for HACEK organisms

C. Perform identification testing by automation

D. Take extra safe precautions for possible Brucella

D

Quiz 2

100

What medium is used to isolate Legionella?BYC

BCYE

200

What is the name of the two diseases caused by Legionella species?

Legionnaire's Disease and Pontiac Fever

300

Gram negative bacillus

Non-lactose fermentor

Oxidase negative

Urease positive

Motile - Swarmer

K/A, gas, H2S

Indole negative

Proteus mirabilis

400

Characteristics of the HACEK group of bacteria include?

A. assocation with UTIs

B. Gram stain of pleomorphic GP bacilli

C. Requirement for 5-10% CO2 for growth

D. Requirement of 42 degrees C for growth

C

500

What organism plays a role in Guillain- Barre syndrome?

A. Helicobacter

B. Aeromonas

C. Campylobacter

D. Plesiomonas

C

Clostridium

100

Gram positive bacilli

Spore-forming, spore shaped like a “tennis racket”

Obligate anaerobe

Motile

Clostridium tetani

200

Gram positive bacillus

Spore-forming

Obligate anaerobe

Motile

Smelly 

Clostridium difficile

300

Gram positive bacillus

Spore-forming

Obligate anaerobe

Motile

Canned food and honey are good homes

Mouse test positive for paralysis/death

Clostridium botulinum

400

Gram positive bacillus

Spore-forming

Obligate anaerobe

Non-motile

Clostrdium perfringens