Microorganisms
Methods used to ensure contaminating organisms introduced into culture materials or the surrounding environment.
Aseptic technique
Step before staining that causes the cells to adhere to the slide.
Smear
Bacteria that grow at a pH range 5-8.5
Organisms used as indicator organisms when testing water quality
Coliform Bacteria
When completing a standard plate count, this is what colonies are reported as.
Colony Forming Units
Bacteriocide
Stain technique used to observe endospores
Spore staining
This color band appears at the top of the medium upon addition of Kovac's reagent, indicating Indole formation
Red
Bacteriophage life stage involving phage replication and destruction of bacterial cells
Lytic
The color of bacterial colonies on MacConkey's agar that are able to ferment lactose
Pink
Type of light microscopy where light is reflected from the specimen, resulting in a dark background and a bright specimen.
Dark-field microscopy
Fatty acid that prevents cells from being stained
Mycolic Acid
Bacteria that grow throughout the tube exclusively using anaerobic respiration. They are unaffected by oxygen levels.
Aerotolerant Anaerobes
Bacteriophage life cycle involving the incorporation of phage DNA into the bacterial chromosome.
Lysogenic
The color of the precipitate in SIM media that is evidence for sulfide production
Black
The three domains of life
Bacteria, Archaea, Eukarya
Stain technique used for delicate bacteria and results in a dark background.
Negative Stain
Molecular lesions formed from a covalent bond between bases in DNA as a result of UV exposure
Dimer or Pyrimidine Dimer
Three tests used to evaluate water quality
Presumptive, Confirmed, Completed
Resistant asexual spore that develops in specific bacterial cells
Endospores
The four supergroups subdividing Eukarya
Unikonta, Excavata, SAR and Archaeplastida
This happens when the decolorization step is skipped.
Gram negative cells appear purple
Bacteria that have an optimal growth of 0 to 30 degrees C
Psychrotrophs
The color of E. coli colonies streaked onto an EMB plate.
Nucleated centers and metallic green sheen
This is the repair system that removes dimers in DNA and inserts new molecules in their place
SOS Emergency repair/Nucleotide Excision Repair