Counting bacteria
Counting cells by growing cells taken from a culture by spreading them on an agar plate
What is spread plating?
Bacterial species that require oxygen to grow
What are obligate aerobes?
Extreme heat loving microorganisms
What is a hyperthermophile?
Organisms which can acquire carbon from atmospheric carbon dioxide
What is an autotroph?
Type of microbe that would be found growing in the hot springs at Yellowstone
What is a hyperthermophiles/thermophiles?
Any counting method which will only identify the cells which are living
What is a viable counting method?
What are bacterial species that can only grow in environments that lack oxygen
What are obligate anaerobes
Microorganisms that must grow at extremely high pressures
What is a barophile?
Organisms that must consume organic compounds to acquire carbon
What are heterotrophs?
Microbes which grow best at room temperature or body temperature
What are mesophiles?
A chamber used to count bacterial cells directly with a light microscope using a grid
What is a Petroff-Hauser chamber?
Bacterial species that are not killed by oxygen but cannot use oxygen metabolically
What are aerotolerant anaerobes?
Bacterium that lives in the human stomach, causes ulcers, and infects approximately 50% of people
What is Helicobacter pylorii?
Microbes that acquire their energy from light
What are phototrophs?
Microbes that grow best in extreme cold
What are psychrophiles?
Using turbidity to measure growth by observing how much light is absorbed by a culture
What is optical density?
Bacterial species that require a small amount of oxygen to grow but cannot grow at high O2 concentrations?
What are microaerophiles
Salt loving microorganisms
What are halophiles?
Microbes that acquire energy from chemical compounds
What are chemotrophs?
Microbes that grow best at room temperature but can grow at low temperatures (approximately 0 degrees C)
What are psychrotrophs?
Despite a decrease in the number of viable cells during the death phase, the wet weight of a growing colony of bacteria will not decrease
What is a downside to the wet weight method for cell enumeration?
Bacterial species that can grow in the presence or absence of oxygen but grows fastest when oxygen is present
What is a falcultative anaerobe?
Base-living microorganisms
What are alkaliphiles?
What are selective agars?
A microbe temperature requirement most likely to cause food spoilage in the refrigerator
What are psychrotrophs?