True/False
Plates such as the MSA, EMB, etc. can only be selective.
(False) These plates are both Selective and Differential.
Gram + plate that contains phenol red, NaCl, and Mannitol
Mannitol Salt Agar
A facultative anaerobe is expected to grow where in the tube?
Throughout. Why?
what substance is being observed in the "Indole" part of the SIM test?
tryptophan
A plate with a shimmering green metallic color. What's the organism on the plate.
E. coli
The three types of hemolysis are beta, gamma, and alpha.
True!
What are the two gram + positive plates we learned about?
MSA and PEA
The area that is cleared around an antibiotic disk on a Mueller Hinton plate is called the .
Zone of Inhibiton
it's in the Bile esculin tube. It inhibits gram positive bacteria.
What is bile?
An MSA plate split in two sections. One section is yellow and the section is pink. What organism is on the pink side.
S. aureus
A bacteria that grow on an EMB plate is a gram - bacteria.
Partially true! EMB plate has exceptions ! Remember some species of staph and enterococcus will grow on this plate.
Why does the EMB plate allow you to differentiate?
Lactose fermenters from non-lactose fermenters.
Growth with no change to the blood plate after incubation. What type of hemolysis?
Gamma
This reagent is used to on the starch plate for the starch hydrolysis
Iodine
A tube where the top is pink after the addition of Indole (Kovac's reagent).
SIMS tube.
In the SIM tube, if it turns blue, sulfur was reduced.
False. what's the color? Dark brown-black.
Which plate would you choose to be able tell if you have a species that vigorously ferments lactose?
EMB! This plate will change the color the of the colonies in order to determine lactose fermentation. E.coli - produces green metallic sheen
Other lactose fermenters- dark purple black colonies
What is phenol red?
A pH indicator that responds to changes in Ph.
The catalase test indicator is p-aminodimethyl-aniline that will change color if this enzyme is present.
Cytochrome c oxidase
A plate with antibiotic disks with a zone of inhibition.
Mueller-Hinton plate
A bacterium that hydrolyzes starch will appear clear on the starch plate.
TRUE
What is an EMB plate selective for?
Gram (-) bacteria would grow. Imhibit gram positive bacteria.
If your bacteria did not grow in the citrate tube, is the test inconclusive?
No. Its negative for citrate. Citrate is differential. The tube remains the same or it changes blue.
Selective component in PEA.
Phenylethyl alcohol.
A tube with a small tube inside to collect gas if produced. Name the small tube.
Durhan tube.