Getting the cells you want to view to stick to your slide. Can be done with heat or chemicals
What is fixation
100
The atom used in all organic molecules
What is carbon
100
an organic compound that is needed for survival that can not be synthesized by the microorganism
What is a growth factor?
100
bacteria that prefers a cold environment
What is a psychrophile
200
Ability to distinguish between objects that are close together
What is resolution
200
The first stain, leaves a bluish purple color
What is cyrstal violet
200
Important atom used in cystine and cysteine bonds.
What is Sulfur
200
pH, osmolarity, nutrition, temperature, pressure
What are some examples of growth factors?
200
bacteria that prefers an environment between room temp and body temp
What is a mesophile
300
uses chemicals with heavy metals to stain the organism or the background
What is an Electron Microscope Stain
300
A new dye, usually a pink or red, that stains the Gram negative cells after they go through decolorization. This has no visible effect on Gram positive cells
What is counterstain
300
The growth limiting nutrient
What is nitrogen
300
Need atmospheric oxygen to live
What is an obligate aerobe
300
bacteria with specialized membranes and enzymes that tolerate pressure
What is barophile
400
a special filter that further slows light waves
What is a phase plate
400
Use of this halogen to push the crystal violet dye in to the cells
What is iodine treatment
400
Essential for obligate aerobes, is toxic to obligate anaerobes
What is oxygen
400
must obtain their nutrients from outside sources
What is a heterotroph
400
bacteria that grow under high osmotic pressure, due to high salt content
What is halophile
500
light passes through the specimen into objective lense
What is Bright Field microscopy
500
alcohol or acetone is used to remove the outer lipopolysaccharide layer in Gram negative cells. This has no effect on Gram positive cells
What is decolorization
500
a big part of the energy molecule ATP, is an important atom for the backbone of DNA molecules
What is phosphorous
500
make their own nutrient from either light or chemical compounds