Stains
Gram's iodine is a ____ used during the _____ stain
mordant, gram
Tryptophanase breaks down tryptophan into _____ , ______ , & _______.
indole, pyruvic acid, NH3
True or False: Mannitol-salt agar, KF Strep agar, EMB agar, and LST MUG broth are selective and differential
True
True or False: S. salivarius colonies appear blue and “gum drop” shaped on mitis-salvarius agar.
True
Safranin is used in the _____ stain and ______ stain
gram, endospore
Black precipitate in SIM agar indicates your bacterium expresses ______.
cysteine desulfhydrase
LST-MUG detects ____ and ____.
fecal coliforms AND coliforms
True or False: Turbidity, gas, and fluorescence in LST-MUG broth and growth on EMB agar means your sample is positive for E. coli.
False; colonies must also have a metallic green sheen on EMB agar to be considered positive for E. coli
What stain is Bacillus subtilis the control culture?
Endospore stain
Phenylalanine is broken down into H2O, ______, & _______ by phenylalanine deaminase.
phenylpyruvic acid, NH3
Mannitol-Salts Agar contains what 2 differential ingredients?
mannitol and phenol red
You streak a Mannitol-salt agar plate and after incubation you see growth and the agar around the growth has turned yellow. What bacteria was inoculated?
Staphylococcus aureus
Methylene blue stains Staphylococcus epidermidis, ______ stains _______. Fill in the blanks and name the stain.
Acid-fast, Carbolfuchsin, Mycobacterium phlei
In DC and LDC broth a positive result should be have a _____ LDC tube and _____ DC tube. In the LDC tube, the presence of _____ raises the pH and indicates expression of ____.
purple, yellow, cadaverine, lysine decarboxylase
Triphenyltetrazolium Chloride is differential for ______ and is in _______ agar.
anaerobic metabolism, KF-Strep
You inoculate LST MUG broth and after incubation you see growth and gas. What would you expect to see on the gram and endospore stain of the same bacteria?
Gram negative, vegetative cells only
You are handed a culture of Klebsiella pneumoniae, what stain would you most likely perform? What dyes and additional culture would you need?
Capsule stain, Staphylococcus epidermidis, congo red and maneval’s solution
You are given a test tube with a bright pink solution containing ammonia and CO2, what amino acid was in the tube previously and what enzyme broke it down?
urea, urease
Methylene blue is ______for ______ bacteria and is a component of _____ agar.
selective, gram negative, eosin methylene blue
You are given a bacterium that is gram positive and catalase negative. You streak a mitis-salvarius plate. After incubation, there are small, flat, black bacteria on the mitis salvarius agar. What bacteria were you given?
Enterococcus