Biological Testing pt.1
In the phenol red fermentation broth, a sugar fermented tube shows which color
yellow
After incubation what should be added to the starch hydrolysis plate?
Iodine
The zinc dust will catalyze nitrate to
Nitrite
Which kind of bacterial growth is fairly uniform growth throughout
aerotolerant anaerobes
In the TSI test, a red slant and yellow bottom means
Glucose fermentation only
in the phenol red fermentation broth, how will you know that there was gas produced?
broth will have bubble
urea hydrolyzed by urease turn into....
ammonia and carbon dioxide
after adding reagents A and B to NRT, a positive result will appear ___ meaning bacteria contains nitrate reductase
red
Describes growth throughout, but primarily at the top
faculative anaerobes
In the TSI test, a yellow slant and yellow bottom means
Glucose, lactose and/or sucrose fermentation
in the Methyl red test, if the broth remains red after adding methyl red this shows a (positive/negative) result
Positive
Phenol red turn what color when in alkaline environment in a urease test
fuchsia
If no bubble appear in the NRT, ______ must be added
Reagents A and B
Growth at the bottom only
obligate anaerobes
In the TSI test, What will the test tube look like if only glucose is fermented and a gas is produced
red/yellow with bubble
A negative result in methyl red test means.... (acids present or not)
acids not present
Which part of the SIM test shows the break down of the amino acid tryptophan?
indole
if there is not change after adding reagents A and B in the NRT, what must be added?
Zinc dust
Shows growth at the top only
oligate aerobes
In the TSI test, Glucose and lactose and/or sucrose fermentation; H2S produced means the tube will be
Yellow/yellow with black precipitate
In the phenol red fermentation broth, a tube that has failed to have a reaction will be...
red
Which is used as the pH indicator in the TSI test?
Phenol Red
lipases hydrolyze _______ into glycerol and fatty acids
triglycerides
Growth towards the middle
microaerophiles
In the TSI test, Glucose fermentation only; Gas produced; H2S produced means the tube will be